Detailed information of TA system TA073886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 962814..963541
• Replicon: chromosome
• Accession: NZ_CP020055
• Organism: Escherichia coli strain AR_0069

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: AM448_RS04895
• Protein ID: WP_000547564.1
• Coordinates: 963230..963541 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM448_RS04890
• Protein ID: WP_000126294.1
• Coordinates: 962814..963233 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM448_RS04870	957864..958391	-	528	WP_001078777.1	electron transport protein HydN	-
AM448_RS04875	958540..959550	-	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
AM448_RS04880	959810..961267	+	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
AM448_RS04885	961276..962700	+	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
AM448_RS04890	962814..963233	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
AM448_RS04895	963230..963541	-	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
AM448_RS04900	963715..964476	-	762	WP_080028721.1	hypothetical protein	-
AM448_RS04905	964501..964971	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
AM448_RS04910	964964..965374	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
AM448_RS04915	965371..966138	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
AM448_RS04920	966138..966680	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
AM448_RS04925	966690..968399	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T73886 WP_000547564.1 NZ_CP020055:c963541-963230 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T73886 NZ_CP020055:c963541-963230 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT73886 WP_000126294.1 NZ_CP020055:c963233-962814 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT73886 NZ_CP020055:c963233-962814 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5