Detailed information of TA system TA073867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2386173..2386675
• Replicon: chromosome
• Accession: NZ_CP020053
• Organism: Enterobacter cloacae complex sp. strain AR_0065

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: AM444_RS11755
• Protein ID: WP_032103793.1
• Coordinates: 2386173..2386427 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: AM444_RS11760
• Protein ID: WP_032103791.1
• Coordinates: 2386424..2386675 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM444_RS11720 (AM444_11800)	2381259..2381783	-	525	WP_015570073.1	lipocalin family protein	-
AM444_RS11725 (AM444_11805)	2381901..2382626	+	726	WP_032103799.1	MerR family transcriptional regulator	-
AM444_RS11730 (AM444_11810)	2382726..2383136	+	411	WP_017693985.1	hydroxyisourate hydrolase	-
AM444_RS11735 (AM444_11815)	2383239..2384199	+	961	Protein_2258	DUF523 and DUF1722 domain-containing protein	-
AM444_RS11740 (AM444_11820)	2384346..2385434	+	1089	WP_032103797.1	AAA family ATPase	-
AM444_RS11745 (AM444_11825)	2385507..2385755	+	249	Protein_2260	type VI secretion system tube protein Hcp	-
AM444_RS11750 (AM444_11830)	2385780..2386097	-	318	WP_032103795.1	DUF1493 family protein	-
AM444_RS11755 (AM444_11835)	2386173..2386427	-	255	WP_032103793.1	Txe/YoeB family addiction module toxin	Toxin
AM444_RS11760 (AM444_11840)	2386424..2386675	-	252	WP_032103791.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
AM444_RS11765 (AM444_11845)	2387227..2388477	-	1251	WP_017693988.1	HlyD family efflux transporter periplasmic adaptor subunit	-
AM444_RS11770 (AM444_11850)	2388484..2389233	-	750	WP_023295680.1	hypothetical protein	-
AM444_RS11775 (AM444_11855)	2389230..2391326	-	2097	WP_017383654.1	peptidase domain-containing ABC transporter	-
AM444_RS11780 (AM444_11860)	2391394..2391579	-	186	WP_001196016.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	hcp/tssD	2368017..2386675	18658

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10089.65 Da
• Isoelectric Point: 7.8740

>T73867 WP_032103793.1 NZ_CP020053:c2386427-2386173 [Enterobacter cloacae complex sp.]
VKITWSEEAWEDYLYWQETDKRMVKKINELIKISCRTPFEGIGKPEPLKHNLAGFWSRRITSEHRMVYAITEEAILIAAC
RYHY

Nucleotide

• Length: 255 bp

>T73867 NZ_CP020053:c2386427-2386173 [Enterobacter cloacae complex sp.]
GTGAAGATCACATGGTCTGAAGAGGCATGGGAAGACTACCTCTATTGGCAGGAGACCGATAAGCGCATGGTGAAGAAAAT
CAATGAGCTTATCAAAATCTCCTGTCGCACTCCGTTTGAGGGGATCGGCAAGCCAGAGCCATTAAAACATAACCTGGCTG
GCTTCTGGTCTCGCCGAATCACCTCTGAGCATCGCATGGTCTATGCCATCACAGAAGAAGCGATCCTCATAGCTGCGTGC
CGATATCACTACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9313.55 Da
• Isoelectric Point: 4.7854

>AT73867 WP_032103791.1 NZ_CP020053:c2386675-2386424 [Enterobacter cloacae complex sp.]
MRVISYSEARQNLSATMVQTTEDRAPVLITRQNGESCVLMSLEEYNSLEETAYLMRSPANAKRLMNSIESLKSGGGKERD
IIE

Nucleotide

• Length: 252 bp

>AT73867 NZ_CP020053:c2386675-2386424 [Enterobacter cloacae complex sp.]
ATGCGTGTTATTAGTTACAGTGAAGCCCGACAAAACCTGTCGGCAACAATGGTCCAGACGACGGAAGATCGCGCGCCGGT
GCTTATTACGCGCCAGAACGGTGAATCTTGTGTTCTGATGTCGCTGGAAGAGTACAACTCGCTTGAAGAAACCGCATACC
TGATGCGCTCCCCGGCAAACGCAAAACGTCTGATGAACTCTATCGAGAGCTTGAAATCTGGTGGCGGGAAGGAAAGGGAC
ATTATTGAGTGA