Detailed information of TA system TA073857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 47344..48055
• Replicon: plasmid unitig_3
• Accession: NZ_CP020051
• Organism: Escherichia coli strain AR_0118

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: AM365_RS27955
• Protein ID: WP_000162415.1
• Coordinates: 47344..47646 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM365_RS27960
• Protein ID: WP_000806445.1
• Coordinates: 47717..48055 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM365_RS27925	42363..43043	-	681	WP_000812238.1	hypothetical protein	-
AM365_RS27930	43027..43932	-	906	WP_080028513.1	recombination-associated protein RdgC	-
AM365_RS27935	43992..44645	-	654	WP_000828872.1	hypothetical protein	-
AM365_RS27940	44642..45622	-	981	WP_000046500.1	hypothetical protein	-
AM365_RS27945	45626..46393	-	768	WP_000203293.1	hypothetical protein	-
AM365_RS27950	46390..47181	-	792	WP_000532307.1	hypothetical protein	-
AM365_RS27955	47344..47646	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM365_RS27960	47717..48055	+	339	WP_000806445.1	HigA family addiction module antidote protein	Antitoxin
AM365_RS27970	48334..49062	+	729	WP_080028515.1	hypothetical protein	-
AM365_RS27975	49096..50289	-	1194	WP_001112629.1	hypothetical protein	-
AM365_RS27980	50282..50611	-	330	WP_000542383.1	hypothetical protein	-
AM365_RS27985	50940..51593	-	654	WP_023908597.1	hypothetical protein	-
AM365_RS27990	51862..52773	+	912	WP_080028516.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..91291	91291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T73857 WP_000162415.1 NZ_CP020051:47344-47646 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T73857 NZ_CP020051:47344-47646 [Escherichia coli]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT73857 WP_000806445.1 NZ_CP020051:47717-48055 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT73857 NZ_CP020051:47717-48055 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAACAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3