Detailed information of TA system TA073856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5118..5775
• Replicon: plasmid unitig_1
• Accession: NZ_CP020049
• Organism: Escherichia coli strain AR_0118

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: AM365_RS26155
• Protein ID: WP_000270043.1
• Coordinates: 5118..5468 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM365_RS26160
• Protein ID: WP_000124640.1
• Coordinates: 5473..5775 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM365_RS26115	1592..2089	-	498	WP_000062185.1	hypothetical protein	-
AM365_RS26120	2092..2580	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
AM365_RS26125	2677..3012	+	336	WP_000683477.1	hypothetical protein	-
AM365_RS26130	3027..3497	-	471	WP_001281821.1	hypothetical protein	-
AM365_RS26135	3490..3861	-	372	WP_000516918.1	hypothetical protein	-
AM365_RS26140	3872..4066	-	195	WP_000343597.1	hypothetical protein	-
AM365_RS26150	4407..4955	-	549	WP_001061573.1	hypothetical protein	-
AM365_RS26155	5118..5468	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM365_RS26160	5473..5775	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
AM365_RS26165	5802..6095	-	294	WP_001239998.1	hypothetical protein	-
AM365_RS26170	6184..6456	-	273	WP_001043046.1	HU family DNA-binding protein	-
AM365_RS26175	6514..7041	-	528	WP_004201083.1	thermonuclease family protein	-
AM365_RS28940	7044..7349	-	306	WP_171820680.1	hypothetical protein	-
AM365_RS26185	7261..8118	-	858	WP_001167036.1	hypothetical protein	-
AM365_RS26190	8105..8335	-	231	WP_000972665.1	hypothetical protein	-
AM365_RS26195	8335..8853	-	519	WP_000210757.1	nitrite reductase	-
AM365_RS26200	8850..9296	-	447	WP_000919343.1	hypothetical protein	-
AM365_RS26205	9296..9655	-	360	WP_000422769.1	hypothetical protein	-
AM365_RS26210	9713..10141	-	429	WP_000591076.1	hypothetical protein	-
AM365_RS26215	10175..10645	-	471	Protein_21	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaNDM-1 / rmtC / sul1 / qacE / aac(6')-Ib / blaCMY-6	htpB	1..141885	141885

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T73856 WP_000270043.1 NZ_CP020049:5118-5468 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T73856 NZ_CP020049:5118-5468 [Escherichia coli]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT73856 WP_000124640.1 NZ_CP020049:5473-5775 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT73856 NZ_CP020049:5473-5775 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9