Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2734179..2734681 Replicon chromosome
Accession NZ_CP020025
Organism Escherichia coli strain WB61

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag B1T56_RS14015 Protein ID WP_000767829.1
Coordinates 2734179..2734433 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag B1T56_RS14020 Protein ID WP_001259255.1
Coordinates 2734430..2734681 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B1T56_RS13995 2730672..2732030 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
B1T56_RS14000 2732020..2732082 - 63 WP_010723108.1 membrane protein YoeI -
B1T56_RS14005 2732297..2733226 - 930 WP_000803363.1 LysR family transcriptional regulator -
B1T56_RS14010 2733272..2734096 - 825 WP_000754737.1 SDR family oxidoreductase -
B1T56_RS14015 2734179..2734433 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
B1T56_RS14020 2734430..2734681 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
B1T56_RS14025 2734964..2735014 + 51 WP_001364200.1 his operon leader peptide -
B1T56_RS14035 2735160..2736059 + 900 WP_063815004.1 ATP phosphoribosyltransferase -
B1T56_RS14040 2736065..2737369 + 1305 WP_063815005.1 histidinol dehydrogenase -
B1T56_RS14045 2737366..2738436 + 1071 WP_032171499.1 histidinol-phosphate transaminase -
B1T56_RS14050 2738436..2739503 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T73813 WP_000767829.1 NZ_CP020025:c2734433-2734179 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T73813 NZ_CP020025:c2734433-2734179 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT73813 WP_001259255.1 NZ_CP020025:c2734681-2734430 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT73813 NZ_CP020025:c2734681-2734430 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TGCTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References