Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 968818..969512 Replicon chromosome
Accession NZ_CP020025
Organism Escherichia coli strain WB61

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag B1T56_RS04985 Protein ID WP_001263491.1
Coordinates 969114..969512 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag B1T56_RS04980 Protein ID WP_000554755.1
Coordinates 968818..969111 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B1T56_RS04955 963852..964112 - 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -
B1T56_RS04960 964298..965071 + 774 WP_022645219.1 C40 family peptidase -
B1T56_RS04965 965169..966800 - 1632 Protein_903 flagellar biosynthesis protein FlhA -
B1T56_RS04970 966786..967640 + 855 WP_105178461.1 putative lateral flagellar export/assembly protein LafU -
B1T56_RS04975 967711..968766 + 1056 WP_022645222.1 DNA polymerase IV -
B1T56_RS04980 968818..969111 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
B1T56_RS04985 969114..969512 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
B1T56_RS04990 969522..969974 + 453 WP_023909048.1 GNAT family N-acetyltransferase -
B1T56_RS04995 970165..971304 + 1140 WP_022645224.1 RNA ligase RtcB family protein -
B1T56_RS05000 971301..971915 + 615 WP_022645225.1 peptide chain release factor H -
B1T56_RS05005 971977..972486 + 510 WP_001361775.1 hydrolase -
B1T56_RS05010 972503..972784 - 282 WP_022645226.1 hypothetical protein -
B1T56_RS05015 972793..974250 - 1458 WP_022645227.1 cytosol nonspecific dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T73794 WP_001263491.1 NZ_CP020025:969114-969512 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T73794 NZ_CP020025:969114-969512 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT73794 WP_000554755.1 NZ_CP020025:968818-969111 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT73794 NZ_CP020025:968818-969111 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References