Detailed information of TA system TA073758

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1425042..1425681
• Replicon: chromosome
• Accession: NZ_CP020014
• Organism: Haemophilus influenzae strain 11P6H

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: BVZ63_RS07030
• Protein ID: WP_005650215.1
• Coordinates: 1425042..1425347 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BVZ63_RS07035
• Protein ID: WP_005650217.1
• Coordinates: 1425358..1425681 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVZ63_RS07010	1420472..1422511	-	2040	WP_005687320.1	excinuclease ABC subunit B	-
BVZ63_RS07020	1423126..1424094	-	969	WP_105197835.1	zinc transporter permease subunit ZevB	-
BVZ63_RS07025	1424097..1424717	-	621	WP_005666732.1	zinc transporter binding subunit ZevA	-
BVZ63_RS07030	1425042..1425347	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BVZ63_RS07035	1425358..1425681	+	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
BVZ63_RS07040	1425844..1427514	+	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
BVZ63_RS07045	1427576..1427917	+	342	WP_005652996.1	SirB2 family protein	-
BVZ63_RS07050	1427922..1429892	+	1971	WP_011272701.1	tRNA(Met) cytidine acetyltransferase TmcA	-
BVZ63_RS08935	1429889..1430062	+	174	WP_011272700.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T73758 WP_005650215.1 NZ_CP020014:1425042-1425347 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T73758 NZ_CP020014:1425042-1425347 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT73758 WP_005650217.1 NZ_CP020014:1425358-1425681 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT73758 NZ_CP020014:1425358-1425681 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70