Detailed information of TA system TA073723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1431803..1432442
• Replicon: chromosome
• Accession: NZ_CP020010
• Organism: Haemophilus influenzae strain 67P38H1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: BV131_RS07115
• Protein ID: WP_005650215.1
• Coordinates: 1431803..1432108 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BV131_RS07120
• Protein ID: WP_005650217.1
• Coordinates: 1432119..1432442 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BV131_RS07095	1427232..1429271	-	2040	WP_005687320.1	excinuclease ABC subunit B	-
BV131_RS07105	1429887..1430855	-	969	WP_005657582.1	zinc transporter permease subunit ZevB	-
BV131_RS07110	1430858..1431478	-	621	WP_005650213.1	zinc transporter binding subunit ZevA	-
BV131_RS07115	1431803..1432108	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BV131_RS07120	1432119..1432442	+	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
BV131_RS07125	1432605..1434275	+	1671	WP_050822628.1	energy-dependent translational throttle protein EttA	-
BV131_RS07130	1434337..1434678	+	342	WP_050822626.1	SirB2 family protein	-
BV131_RS07135	1434683..1436527	+	1845	WP_050822624.1	tRNA(Met) cytidine acetyltransferase TmcA	-
BV131_RS09035	1436524..1436697	+	174	WP_005657572.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T73723 WP_005650215.1 NZ_CP020010:1431803-1432108 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T73723 NZ_CP020010:1431803-1432108 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT73723 WP_005650217.1 NZ_CP020010:1432119-1432442 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT73723 NZ_CP020010:1432119-1432442 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70