Detailed information of TA system TA073715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1728372..1729011
• Replicon: chromosome
• Accession: NZ_CP020009
• Organism: Haemophilus influenzae strain 5P54H1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: BV122_RS08745
• Protein ID: WP_005650215.1
• Coordinates: 1728706..1729011 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BV122_RS08740
• Protein ID: WP_005650217.1
• Coordinates: 1728372..1728695 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BV122_RS09445	1723991..1724164	-	174	WP_011272700.1	DUF5363 domain-containing protein	-
BV122_RS08725	1724161..1726131	-	1971	WP_011961904.1	tRNA(Met) cytidine acetyltransferase TmcA	-
BV122_RS08730	1726136..1726477	-	342	WP_005652996.1	SirB2 family protein	-
BV122_RS08735	1726539..1728209	-	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
BV122_RS08740	1728372..1728695	-	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
BV122_RS08745	1728706..1729011	-	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BV122_RS08750	1729336..1729956	+	621	WP_005694310.1	zinc transporter binding subunit ZevA	-
BV122_RS08755	1729959..1730927	+	969	WP_042593950.1	zinc transporter permease subunit ZevB	-
BV122_RS08765	1731542..1733581	+	2040	WP_042599673.1	excinuclease ABC subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T73715 WP_005650215.1 NZ_CP020009:c1729011-1728706 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T73715 NZ_CP020009:c1729011-1728706 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT73715 WP_005650217.1 NZ_CP020009:c1728695-1728372 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT73715 NZ_CP020009:c1728695-1728372 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70