Detailed information of TA system TA073709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1456749..1457388
• Replicon: chromosome
• Accession: NZ_CP020008
• Organism: Haemophilus influenzae strain 5P28H1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: BV121_RS07215
• Protein ID: WP_005650215.1
• Coordinates: 1456749..1457054 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BV121_RS07220
• Protein ID: WP_005650217.1
• Coordinates: 1457065..1457388 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BV121_RS07195	1452179..1454218	-	2040	WP_042599673.1	excinuclease ABC subunit B	-
BV121_RS07205	1454833..1455801	-	969	WP_042593950.1	zinc transporter permease subunit ZevB	-
BV121_RS07210	1455804..1456424	-	621	WP_005694310.1	zinc transporter binding subunit ZevA	-
BV121_RS07215	1456749..1457054	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BV121_RS07220	1457065..1457388	+	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
BV121_RS07225	1457551..1459221	+	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
BV121_RS07230	1459283..1459624	+	342	WP_005652996.1	SirB2 family protein	-
BV121_RS07235	1459629..1461599	+	1971	WP_011961904.1	tRNA(Met) cytidine acetyltransferase TmcA	-
BV121_RS09420	1461596..1461769	+	174	WP_011272700.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T73709 WP_005650215.1 NZ_CP020008:1456749-1457054 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T73709 NZ_CP020008:1456749-1457054 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT73709 WP_005650217.1 NZ_CP020008:1457065-1457388 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT73709 NZ_CP020008:1457065-1457388 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70