Detailed information of TA system TA073703

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1699245..1699884
• Replicon: chromosome
• Accession: NZ_CP020007
• Organism: Haemophilus influenzae strain 48P153H1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: BV085_RS08470
• Protein ID: WP_005650215.1
• Coordinates: 1699579..1699884 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BV085_RS08465
• Protein ID: WP_005650217.1
• Coordinates: 1699245..1699568 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BV085_RS09160	1694864..1695037	-	174	WP_005657572.1	DUF5363 domain-containing protein	-
BV085_RS08450	1695034..1697004	-	1971	WP_005687319.1	tRNA(Met) cytidine acetyltransferase TmcA	-
BV085_RS08455	1697009..1697350	-	342	WP_005652996.1	SirB2 family protein	-
BV085_RS08460	1697412..1699082	-	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
BV085_RS08465	1699245..1699568	-	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
BV085_RS08470	1699579..1699884	-	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BV085_RS08475	1700209..1700829	+	621	WP_005666732.1	zinc transporter binding subunit ZevA	-
BV085_RS08480	1700832..1701800	+	969	WP_005666730.1	zinc transporter permease subunit ZevB	-
BV085_RS08490	1702415..1704454	+	2040	WP_005687320.1	excinuclease ABC subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T73703 WP_005650215.1 NZ_CP020007:c1699884-1699579 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T73703 NZ_CP020007:c1699884-1699579 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT73703 WP_005650217.1 NZ_CP020007:c1699568-1699245 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT73703 NZ_CP020007:c1699568-1699245 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70