Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 49319..49854 Replicon plasmid p268-1
Accession NZ_CP019993
Organism Enterococcus faecium isolate 2014-VREF-268

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag B4W81_RS15565 Protein ID WP_002314366.1
Coordinates 49582..49854 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag B4W81_RS15560 Protein ID WP_002297422.1
Coordinates 49319..49582 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4W81_RS15535 45322..45594 + 273 WP_002305107.1 DUF3884 family protein -
B4W81_RS15540 45615..46514 + 900 WP_002303235.1 aldose 1-epimerase family protein -
B4W81_RS15550 48228..48791 + 564 Protein_52 IS6 family transposase -
B4W81_RS15555 48797..49213 + 417 WP_002303153.1 hypothetical protein -
B4W81_RS15560 49319..49582 + 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
B4W81_RS15565 49582..49854 + 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
B4W81_RS15570 49931..50260 + 330 WP_002292678.1 AbrB family transcriptional regulator -
B4W81_RS15575 50250..50536 + 287 Protein_57 type II toxin-antitoxin system RelE/ParE family toxin -
B4W81_RS15580 50822..51679 + 858 WP_002292680.1 AAA family ATPase -
B4W81_RS15585 51680..52228 + 549 WP_002292681.1 hypothetical protein -
B4W81_RS15590 52488..52843 + 356 Protein_60 hypothetical protein -
B4W81_RS15595 52898..53578 - 681 WP_002331383.1 IS6-like element IS1216 family transposase -
B4W81_RS15600 53654..54297 - 644 Protein_62 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(2'')-Ia / aac(6')-aph(2'') - 1..243818 243818
- inside IScluster/Tn - - 46986..64609 17623


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T73670 WP_002314366.1 NZ_CP019993:49582-49854 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T73670 NZ_CP019993:49582-49854 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT73670 WP_002297422.1 NZ_CP019993:49319-49582 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT73670 NZ_CP019993:49319-49582 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References