Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 262462..262997 Replicon plasmid p63-1
Accession NZ_CP019989
Organism Enterococcus faecium isolate 2014-VREF-63

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag B4W80_RS16235 Protein ID WP_002314366.1
Coordinates 262725..262997 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag B4W80_RS16230 Protein ID WP_002297422.1
Coordinates 262462..262725 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4W80_RS16215 257890..260139 + 2250 WP_002314387.1 alpha-galactosidase -
B4W80_RS16220 260142..261317 + 1176 WP_002336205.1 MFS transporter -
B4W80_RS16225 261320..262228 - 909 Protein_284 IS982 family transposase -
B4W80_RS16230 262462..262725 + 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
B4W80_RS16235 262725..262997 + 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
B4W80_RS16240 263074..263403 + 330 WP_002292678.1 AbrB family transcriptional regulator -
B4W80_RS16245 263393..263680 + 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
B4W80_RS16250 263964..264821 + 858 WP_002292680.1 AAA family ATPase -
B4W80_RS16255 264822..265370 + 549 WP_002292681.1 hypothetical protein -
B4W80_RS16260 265630..265985 + 356 Protein_291 hypothetical protein -
B4W80_RS16265 266040..266720 - 681 WP_002331383.1 IS6-like element IS1216 family transposase -
B4W80_RS16270 266796..267439 - 644 Protein_293 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / aac(6')-aph(2'') - 1..287502 287502
- inside IScluster/Tn - - 251861..276419 24558


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T73668 WP_002314366.1 NZ_CP019989:262725-262997 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T73668 NZ_CP019989:262725-262997 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT73668 WP_002297422.1 NZ_CP019989:262462-262725 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT73668 NZ_CP019989:262462-262725 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References