Detailed information of TA system TA073660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1311380..1311930
• Replicon: chromosome
• Accession: NZ_CP019986
• Organism: Citrobacter werkmanii strain BF-6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: B2G73_RS06565
• Protein ID: WP_079224183.1
• Coordinates: 1311664..1311930 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B2G73_RS06560
• Protein ID: WP_079224181.1
• Coordinates: 1311380..1311664 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B2G73_RS06545	1309075..1309956	+	882	WP_061381322.1	formate dehydrogenase N subunit beta	-
B2G73_RS06550	1309949..1310605	+	657	WP_016153106.1	formate dehydrogenase-N subunit gamma	-
B2G73_RS06555	1310733..1311332	+	600	WP_061381323.1	inorganic diphosphatase	-
B2G73_RS06560	1311380..1311664	-	285	WP_079224181.1	HigA family addiction module antidote protein	Antitoxin
B2G73_RS06565	1311664..1311930	-	267	WP_079224183.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B2G73_RS06570	1312139..1313854	-	1716	WP_042308361.1	ATP-binding cassette domain-containing protein	-
B2G73_RS06575	1314273..1315175	+	903	WP_079224185.1	endonuclease/exonuclease/phosphatase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-88)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-86)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10258.81 Da
• Isoelectric Point: 10.1807

>T73660 WP_079224183.1 NZ_CP019986:c1311930-1311664 [Citrobacter werkmanii]
MIMNFRHKGLRDLFLHGRTSGVMAKHVRRLRHRLAASKVTDINMPGYKLHSLIGERDGVWAISVSGNWRITFEFVNGDAY
ILDYEDYH

Nucleotide

• Length: 267 bp

>T73660 NZ_CP019986:c1311930-1311664 [Citrobacter werkmanii]
ATGATCATGAACTTCAGACACAAAGGATTGCGTGACTTGTTTCTTCATGGGCGAACATCAGGCGTTATGGCGAAGCATGT
CCGACGATTACGACACAGACTTGCGGCAAGTAAGGTTACAGATATCAATATGCCCGGTTATAAGCTTCATTCTCTTATAG
GCGAGCGTGATGGAGTTTGGGCAATTTCTGTTTCAGGTAACTGGCGTATTACGTTTGAATTCGTCAATGGCGACGCGTAC
ATACTGGACTACGAGGACTATCATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10323.98 Da
• Isoelectric Point: 8.8094

>AT73660 WP_079224181.1 NZ_CP019986:c1311664-1311380 [Citrobacter werkmanii]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLAGKAALTPEMAIKLSVVIGSSPEIWLNLQNTWSLAEAQ
KNVDTSRLRKLAVQ

Nucleotide

• Length: 285 bp

>AT73660 NZ_CP019986:c1311664-1311380 [Citrobacter werkmanii]
ATGAAAATGGCAAATCATCCCCGCCCTGGGGATATCATTCAGGAAGCACTGGACGAACTCAATGTCAGTCTGCGCGAATT
TGCAAGGGCGATGGAGATTGCACCTTCGACGGCAAGCCGACTATTAGCCGGGAAAGCGGCCCTCACGCCTGAGATGGCGA
TCAAACTCTCTGTCGTAATTGGTAGTTCACCCGAAATATGGTTAAACCTGCAAAATACCTGGAGCCTGGCAGAGGCCCAA
AAAAACGTGGATACTTCACGCCTGCGCAAACTGGCAGTGCAGTGA