Detailed information of TA system TA073634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4760619..4761214
• Replicon: chromosome
• Accession: NZ_CP019967
• Organism: Rhodopseudomonas palustris strain YSC3

Toxin (Protein)

• Gene name: graT
• Uniprot ID: B3Q7W1
• Locus tag: RPYSC3_RS21950
• Protein ID: WP_012497552.1
• Coordinates: 4760619..4760897 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RPYSC3_RS21955
• Protein ID: WP_012497553.1
• Coordinates: 4760909..4761214 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RPYSC3_RS21940	4757021..4757377	-	357	WP_107356885.1	hypothetical protein	-
RPYSC3_RS21945	4757495..4760560	+	3066	WP_107356886.1	excinuclease ABC subunit UvrB	-
RPYSC3_RS21950	4760619..4760897	+	279	WP_012497552.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RPYSC3_RS21955	4760909..4761214	+	306	WP_012497553.1	HigA family addiction module antidote protein	Antitoxin
RPYSC3_RS21960	4761321..4761626	-	306	WP_199851767.1	(2Fe-2S) ferredoxin domain-containing protein	-
RPYSC3_RS21965	4762053..4763306	-	1254	WP_107356887.1	acetyl-CoA C-acetyltransferase	-
RPYSC3_RS21970	4763520..4763912	+	393	WP_107356888.1	hypothetical protein	-
RPYSC3_RS21975	4764397..4764828	+	432	WP_107356889.1	nuclear transport factor 2 family protein	-
RPYSC3_RS21980	4765036..4765476	+	441	WP_107345926.1	hypothetical protein	-
RPYSC3_RS25205	4765616..4765762	-	147	WP_199851768.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10913.43 Da
• Isoelectric Point: 9.0805

>T73634 WP_012497552.1 NZ_CP019967:4760619-4760897 [Rhodopseudomonas palustris]
MIRSFRNPATARLFADEDVPRFRAIERQARRKLLLLDGAAKLDDLRQPPGNRLEALKGDRRGQYSIRINDQWRICFEWRE
DGAEMVEIVDYH

Nucleotide

• Length: 279 bp

>T73634 NZ_CP019967:4760619-4760897 [Rhodopseudomonas palustris]
ATGATCCGCTCGTTCCGAAATCCGGCGACCGCGCGGCTGTTTGCTGATGAGGACGTGCCGCGGTTTCGCGCGATCGAACG
GCAAGCTCGTCGAAAGCTGCTGCTCCTGGACGGCGCCGCCAAACTCGACGATTTGCGGCAACCTCCGGGCAACAGGCTCG
AGGCCTTGAAGGGCGATCGCCGCGGCCAATACTCGATCCGCATCAACGATCAATGGCGGATTTGTTTCGAGTGGCGTGAG
GACGGCGCAGAAATGGTCGAAATCGTGGACTATCATTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11312.14 Da
• Isoelectric Point: 10.2943

>AT73634 WP_012497553.1 NZ_CP019967:4760909-4761214 [Rhodopseudomonas palustris]
MAKPKQLPPIPPGEILIEEFMRPNGISQNRLARDIDINPARVNDIVHGRSAITAAVALRLAKYFGTTPELWMNLQASYDL
RRARAADWPAIEPRVRVLAAE

Nucleotide

• Length: 306 bp

>AT73634 NZ_CP019967:4760909-4761214 [Rhodopseudomonas palustris]
ATGGCCAAACCAAAGCAGCTCCCCCCGATCCCTCCCGGCGAGATTCTGATCGAGGAATTCATGCGCCCGAACGGGATCAG
TCAGAACCGGCTGGCGCGCGATATCGACATCAATCCGGCCCGGGTCAACGACATCGTGCACGGCCGCTCTGCGATCACCG
CCGCCGTCGCCCTGCGGCTGGCGAAGTACTTCGGCACGACGCCCGAACTCTGGATGAACCTGCAGGCGTCCTACGACCTG
CGCCGCGCCCGCGCCGCAGACTGGCCCGCGATCGAGCCGCGCGTCCGCGTGCTCGCCGCGGAGTAG

Structures

Toxin

• AlphaFold DB: A0A2R4GZL3