Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2433014..2433741 Replicon chromosome
Accession NZ_CP019961
Organism Escherichia coli strain HKUOPY1

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag B2J87_RS12530 Protein ID WP_000550189.1
Coordinates 2433014..2433328 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6D0C7R9
Locus tag B2J87_RS12535 Protein ID WP_060707500.1
Coordinates 2433325..2433741 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B2J87_RS12505 2429172..2430158 - 987 WP_001301393.1 Gfo/Idh/MocA family oxidoreductase -
B2J87_RS12510 2430237..2430929 - 693 WP_001411307.1 vancomycin high temperature exclusion protein -
B2J87_RS12515 2431006..2431509 - 504 WP_001401153.1 M48 family metallopeptidase -
B2J87_RS12520 2431594..2432730 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
B2J87_RS12530 2433014..2433328 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
B2J87_RS12535 2433325..2433741 + 417 WP_060707500.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
B2J87_RS12540 2433786..2435804 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
B2J87_RS12545 2436030..2438381 - 2352 WP_053295378.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T73610 WP_000550189.1 NZ_CP019961:2433014-2433328 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T73610 NZ_CP019961:2433014-2433328 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15067.48 Da        Isoelectric Point: 4.3797

>AT73610 WP_060707500.1 NZ_CP019961:2433325-2433741 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSEKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT73610 NZ_CP019961:2433325-2433741 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGAGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure
AlphaFold DB A0A6D0C7R9

References