Detailed information of TA system TA073607

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 756959..757653
• Replicon: chromosome
• Accession: NZ_CP019961
• Organism: Escherichia coli strain HKUOPY1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: B2J87_RS04125
• Protein ID: WP_001263493.1
• Coordinates: 756959..757357 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: B2J87_RS04130
• Protein ID: WP_000554757.1
• Coordinates: 757360..757653 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B2J87_RS04095	751959..753203	-	1245	WP_000189541.1	esterase FrsA	-
-	752619..752699	-	81	NuclAT_12	-	-
-	752619..752699	-	81	NuclAT_12	-	-
-	752619..752699	-	81	NuclAT_12	-	-
-	752619..752699	-	81	NuclAT_12	-	-
B2J87_RS04100	753295..753753	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
B2J87_RS04105	754014..755471	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
B2J87_RS04110	755528..756049	-	522	Protein_735	peptide chain release factor H	-
B2J87_RS04115	756048..756251	-	204	Protein_736	RNA ligase RtcB family protein	-
B2J87_RS04120	756497..756949	-	453	WP_107316937.1	GNAT family N-acetyltransferase	-
B2J87_RS04125	756959..757357	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
B2J87_RS04130	757360..757653	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
B2J87_RS04135	757705..758760	-	1056	WP_001226164.1	DNA polymerase IV	-
B2J87_RS04140	758831..759616	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
B2J87_RS04145	759588..761300	+	1713	Protein_742	flagellar biosynthesis protein FlhA	-
B2J87_RS04150	761516..762013	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T73607 WP_001263493.1 NZ_CP019961:c757357-756959 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T73607 NZ_CP019961:c757357-756959 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT73607 WP_000554757.1 NZ_CP019961:c757653-757360 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT73607 NZ_CP019961:c757653-757360 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6