Detailed information of TA system TA073593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4515186..4515788
• Replicon: chromosome
• Accession: NZ_CP019953
• Organism: Escherichia coli M8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: B2H83_RS22405
• Protein ID: WP_000897305.1
• Coordinates: 4515186..4515497 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B2H83_RS22410
• Protein ID: WP_000356397.1
• Coordinates: 4515498..4515788 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B2H83_RS22380	4511100..4511699	+	600	WP_001295269.1	glucose-1-phosphatase	-
B2H83_RS22385	4511693..4512565	+	873	WP_000920762.1	virulence factor BrkB family protein	-
B2H83_RS22390	4512562..4512999	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
B2H83_RS22395	4513044..4513985	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
B2H83_RS22400	4514049..4514957	-	909	WP_001162704.1	alpha/beta hydrolase	-
B2H83_RS22405	4515186..4515497	+	312	WP_000897305.1	hypothetical protein	Toxin
B2H83_RS22410	4515498..4515788	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
B2H83_RS22415	4516393..4516611	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
B2H83_RS22420	4516830..4517072	+	243	WP_001087409.1	hypothetical protein	-
B2H83_RS22425	4517402..4518331	-	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
B2H83_RS22430	4518328..4518963	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
B2H83_RS22435	4518960..4519862	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T73593 WP_000897305.1 NZ_CP019953:4515186-4515497 [Escherichia coli M8]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T73593 NZ_CP019953:4515186-4515497 [Escherichia coli M8]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT73593 WP_000356397.1 NZ_CP019953:4515498-4515788 [Escherichia coli M8]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT73593 NZ_CP019953:4515498-4515788 [Escherichia coli M8]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7