Detailed information of TA system TA073591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3717481..3718280
• Replicon: chromosome
• Accession: NZ_CP019953
• Organism: Escherichia coli M8

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F4VJD3
• Locus tag: B2H83_RS18475
• Protein ID: WP_000347266.1
• Coordinates: 3717816..3718280 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: B2H83_RS18470
• Protein ID: WP_001307405.1
• Coordinates: 3717481..3717816 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B2H83_RS18455	3713266..3714036	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
B2H83_RS18460	3714052..3715386	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
B2H83_RS18465	3715761..3717332	+	1572	WP_001273741.1	galactarate dehydratase	-
B2H83_RS18470	3717481..3717816	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
B2H83_RS18475	3717816..3718280	+	465	WP_000347266.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
B2H83_RS18480	3718335..3719144	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
B2H83_RS18485	3719393..3720673	+	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
B2H83_RS18490	3720696..3721169	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
B2H83_RS18495	3721180..3721959	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
B2H83_RS18500	3721949..3722827	+	879	WP_001315856.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
B2H83_RS18505	3722845..3723279	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3708220..3718280	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17841.18 Da
• Isoelectric Point: 9.4947

>T73591 WP_000347266.1 NZ_CP019953:3717816-3718280 [Escherichia coli M8]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T73591 NZ_CP019953:3717816-3718280 [Escherichia coli M8]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT73591 WP_001307405.1 NZ_CP019953:3717481-3717816 [Escherichia coli M8]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT73591 NZ_CP019953:3717481-3717816 [Escherichia coli M8]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A836NGD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7