Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2399896..2400398 Replicon chromosome
Accession NZ_CP019953
Organism Escherichia coli M8

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag B2H83_RS12075 Protein ID WP_000767829.1
Coordinates 2399896..2400150 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag B2H83_RS12080 Protein ID WP_001259255.1
Coordinates 2400147..2400398 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B2H83_RS12045 2394911..2395138 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
B2H83_RS12050 2395152..2396210 - 1059 WP_000492346.1 transport protein YeeE -
B2H83_RS12055 2396389..2397747 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
B2H83_RS12060 2397737..2397799 - 63 WP_010723108.1 membrane protein YoeI -
B2H83_RS12065 2398014..2398943 - 930 WP_000803351.1 LysR family transcriptional regulator -
B2H83_RS12070 2398989..2399813 - 825 WP_000754749.1 SDR family oxidoreductase -
B2H83_RS12075 2399896..2400150 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
B2H83_RS12080 2400147..2400398 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
B2H83_RS12085 2400681..2400731 + 51 WP_001364200.1 his operon leader peptide -
B2H83_RS12090 2400877..2401776 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
B2H83_RS12095 2401782..2403086 + 1305 WP_001369878.1 histidinol dehydrogenase -
B2H83_RS12100 2403083..2404153 + 1071 WP_000108962.1 histidinol-phosphate transaminase -
B2H83_RS12105 2404153..2405220 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - ugd / gnd / galF / wcaJ / manB / wcaG / gmd / wza 2391051..2592008 200957


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T73586 WP_000767829.1 NZ_CP019953:c2400150-2399896 [Escherichia coli M8]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T73586 NZ_CP019953:c2400150-2399896 [Escherichia coli M8]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT73586 WP_001259255.1 NZ_CP019953:c2400398-2400147 [Escherichia coli M8]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT73586 NZ_CP019953:c2400398-2400147 [Escherichia coli M8]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References