Detailed information of TA system TA073484

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53809..54063
• Replicon: plasmid unnamed3
• Accession: NZ_CP019909
• Organism: Escherichia coli strain MDR_56

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: B1200_RS28580
• Protein ID: WP_001351576.1
• Coordinates: 53809..54015 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	54002..54063 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1200_RS28535	49352..49699	+	348	Protein_51	IS1-like element IS1A family transposase	-
B1200_RS28545	50139..50426	-	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	-
B1200_RS28550	50423..50674	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
B1200_RS28555	51637..52494	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
B1200_RS28560	52487..52969	-	483	WP_001273588.1	hypothetical protein	-
B1200_RS28565	52962..53036	-	75	WP_001442103.1	RepA leader peptide Tap	-
B1200_RS28575	53268..53525	-	258	WP_000083845.1	replication regulatory protein RepA	-
B1200_RS28580	53809..54015	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	54002..54063	+	62	NuclAT_1	-	Antitoxin
-	54002..54063	+	62	NuclAT_1	-	Antitoxin
-	54002..54063	+	62	NuclAT_1	-	Antitoxin
-	54002..54063	+	62	NuclAT_1	-	Antitoxin
B1200_RS30395	54319..54393	-	75	Protein_59	endonuclease	-
B1200_RS28590	54639..54851	-	213	WP_001299730.1	ANR family transcriptional regulator	-
B1200_RS28595	54987..55547	-	561	WP_000139315.1	fertility inhibition protein FinO	-
B1200_RS28600	55650..56510	-	861	WP_000704513.1	alpha/beta hydrolase	-
B1200_RS28605	56569..57315	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..85009	85009

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T73484 WP_001351576.1 NZ_CP019909:c54015-53809 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T73484 NZ_CP019909:c54015-53809 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT73484 NZ_CP019909:54002-54063 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin