Detailed information of TA system TA073472

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 129820..130084
• Replicon: plasmid unnamed6
• Accession: NZ_CP019905
• Organism: Escherichia coli strain MDR_56

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: B1200_RS27585
• Protein ID: WP_001303307.1
• Coordinates: 129820..129972 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	130022..130084 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1200_RS27555	125664..126326	+	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-
B1200_RS27560	126424..126633	+	210	WP_032178830.1	hemolysin expression modulator Hha	-
B1200_RS27565	126965..127369	-	405	WP_001175009.1	IS200/IS605 family transposase	-
B1200_RS27570	127428..128654	+	1227	WP_024186316.1	transposase	-
B1200_RS30355	128699..128836	+	138	WP_047088497.1	hypothetical protein	-
B1200_RS27580	129497..129748	+	252	WP_001291965.1	hypothetical protein	-
B1200_RS27585	129820..129972	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
-	130022..130084	+	63	NuclAT_0	-	Antitoxin
-	130022..130084	+	63	NuclAT_0	-	Antitoxin
-	130022..130084	+	63	NuclAT_0	-	Antitoxin
-	130022..130084	+	63	NuclAT_0	-	Antitoxin
B1200_RS27590	130264..131472	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
B1200_RS29850	131491..131712	+	222	Protein_150	DsbC family protein	-
B1200_RS27605	133129..133872	+	744	WP_080087028.1	DsbC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSHV-12 / dfrA12 / aadA2 / qacE / sul1 / mph(A) / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / aph(3')-IIa	-	1..142304	142304
	-	inside	IScluster/Tn	-	-	126965..132966	6001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T73472 WP_001303307.1 NZ_CP019905:c129972-129820 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T73472 NZ_CP019905:c129972-129820 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT73472 NZ_CP019905:130022-130084 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin