Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4070606..4071108 Replicon chromosome
Accession NZ_CP019903
Organism Escherichia coli strain MDR_56

Toxin (Protein)


Gene name yoeB Uniprot ID A0A8E0FJZ4
Locus tag B1200_RS21565 Protein ID WP_000767833.1
Coordinates 4070606..4070860 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag B1200_RS21570 Protein ID WP_001259255.1
Coordinates 4070857..4071108 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B1200_RS21540 4065621..4065848 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
B1200_RS21545 4065862..4066920 - 1059 WP_000492305.1 transport protein YeeE -
B1200_RS21550 4067099..4068457 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
B1200_RS29735 4068447..4068509 - 63 WP_010723108.1 membrane protein YoeI -
B1200_RS21555 4068724..4069653 - 930 WP_000803351.1 LysR family transcriptional regulator -
B1200_RS21560 4069699..4070523 - 825 WP_032202495.1 SDR family oxidoreductase -
B1200_RS21565 4070606..4070860 - 255 WP_000767833.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
B1200_RS21570 4070857..4071108 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
B1200_RS21575 4071390..4071440 + 51 WP_001364200.1 his operon leader peptide -
B1200_RS21585 4071586..4072485 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
B1200_RS21590 4072491..4073795 + 1305 WP_072652220.1 histidinol dehydrogenase -
B1200_RS21595 4073792..4074862 + 1071 WP_000108969.1 histidinol-phosphate transaminase -
B1200_RS21600 4074862..4075929 + 1068 WP_072652219.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10173.54 Da        Isoelectric Point: 8.0353

>T73466 WP_000767833.1 NZ_CP019903:c4070860-4070606 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T73466 NZ_CP019903:c4070860-4070606 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAATCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT73466 WP_001259255.1 NZ_CP019903:c4071108-4070857 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT73466 NZ_CP019903:c4071108-4070857 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References