Detailed information of TA system TA073446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1283787..1284389
• Replicon: chromosome
• Accession: NZ_CP019903
• Organism: Escherichia coli strain MDR_56

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: B1200_RS06710
• Protein ID: WP_000897305.1
• Coordinates: 1283787..1284098 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B1200_RS06715
• Protein ID: WP_000356397.1
• Coordinates: 1284099..1284389 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1200_RS06685	1279701..1280300	+	600	WP_001315111.1	glucose-1-phosphatase	-
B1200_RS06690	1280294..1281166	+	873	WP_000920762.1	virulence factor BrkB family protein	-
B1200_RS06695	1281163..1281600	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
B1200_RS06700	1281645..1282586	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
B1200_RS06705	1282650..1283558	-	909	WP_001318161.1	alpha/beta hydrolase	-
B1200_RS06710	1283787..1284098	+	312	WP_000897305.1	hypothetical protein	Toxin
B1200_RS06715	1284099..1284389	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
B1200_RS06720	1284994..1285212	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
B1200_RS06725	1285427..1286356	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
B1200_RS06730	1286353..1286988	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
B1200_RS06735	1286985..1287887	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T73446 WP_000897305.1 NZ_CP019903:1283787-1284098 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T73446 NZ_CP019903:1283787-1284098 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT73446 WP_000356397.1 NZ_CP019903:1284099-1284389 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT73446 NZ_CP019903:1284099-1284389 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7