Detailed information of TA system TA073419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1161661..1162388
• Replicon: chromosome
• Accession: NZ_CP019889
• Organism: Enterobacter hormaechei strain FRM

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: B1023_RS05620
• Protein ID: WP_045348581.1
• Coordinates: 1162077..1162388 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8B3V464
• Locus tag: B1023_RS05615
• Protein ID: WP_022650546.1
• Coordinates: 1161661..1162080 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1023_RS05605	1159491..1160612	-	1122	WP_017382544.1	glutamate--cysteine ligase	-
B1023_RS05610	1160718..1161608	+	891	WP_045348582.1	oxidoreductase	-
B1023_RS05615	1161661..1162080	-	420	WP_022650546.1	helix-turn-helix domain-containing protein	Antitoxin
B1023_RS05620	1162077..1162388	-	312	WP_045348581.1	type II toxin-antitoxin system HigB family toxin	Toxin
B1023_RS05625	1162787..1163431	+	645	WP_045348580.1	leucine efflux protein LeuE	-
B1023_RS05630	1163550..1164152	+	603	WP_045348579.1	tyrosine-type recombinase/integrase	-
B1023_RS05635	1164289..1164957	+	669	WP_023296073.1	epoxyqueuosine reductase QueH	-
B1023_RS05640	1165190..1165375	-	186	Protein_1067	hypothetical protein	-
B1023_RS05645	1165665..1166546	-	882	WP_003858850.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-134)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12562.47 Da
• Isoelectric Point: 9.9435

>T73419 WP_045348581.1 NZ_CP019889:c1162388-1162077 [Enterobacter hormaechei]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPLPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKI
FIKHISTHADYDKLMDYYRRTKE

Nucleotide

• Length: 312 bp

>T73419 NZ_CP019889:c1162388-1162077 [Enterobacter hormaechei]
ATGCACATAGTCTCAAGAGCACCTTTCGACACAGCGGCCAGACATTATCCCAATCAAGCACAAGCCCTGGACGATCTTTA
CCGGGTACTAAAAAAGGAACACTACCCCTTACCAGACGAGATGCGAAAGCGCTTTCCCAGCCTGGACCGGATGAAATACC
GGGAAAAATGGTGGGTCATTGATGTGGGTGGCGGGCATCTAAGAGTAATGTTTTTTGCTGATTTTGAGCGGGGAAAGATC
TTCATCAAACACATCAGCACTCACGCAGACTATGACAAGCTCATGGACTACTACCGGAGAACGAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15447.51 Da
• Isoelectric Point: 4.6051

>AT73419 WP_022650546.1 NZ_CP019889:c1162080-1161661 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Nucleotide

• Length: 420 bp

>AT73419 NZ_CP019889:c1162080-1161661 [Enterobacter hormaechei]
ATGAATTATGCAGCAGCCATCAAAGCGGCAAACGCTCTGACAAACGAACTCCCTTTCCTGGGTTCAAGCCCGTCGCGGCA
AGACTACGAGGATGCGCTGGCGCTGGTGGAGTATCTTATCGAGCACGACCCGGATAACCCGCTGGTAGAGATGCTTACTG
CCAAAATCGATAAGTACGAAAACGAGTCCCCGGAGTTTGCAGAATTTAACGCGCGTATTGCCAGTATTCCGTCAGGCGTT
GCGTTACTGCGAACGCTGATGGATCAGTACCAACTCACTCAGAGTGATTTTGAGAACGAGATTGGTAAGAAGTCTCTGGT
GAGTCGGATCCTGAACGGACAGCGCACCTTAACGCTGGATCATATGCGCGCGCTGGCGAAGCGTTTTGGTTTGCCGGTGA
GTGCGTTTGTAGGGAACTGA

Structures

Antitoxin

• AlphaFold DB: A0A8B3V464