Detailed information of TA system TA073399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 206740..207333
• Replicon: chromosome
• Accession: NZ_CP019871
• Organism: Pseudomonas syringae pv. tomato strain B13-200

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: XJ28_RS01080
• Protein ID: WP_007246530.1
• Coordinates: 206740..207018 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: Q88B19
• Locus tag: XJ28_RS01085
• Protein ID: WP_005739893.1
• Coordinates: 207028..207333 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XJ28_RS01060	202003..203241	-	1239	WP_007246526.1	diaminopimelate decarboxylase	-
XJ28_RS01065	203243..204118	-	876	WP_007246527.1	DMT family transporter	-
XJ28_RS01070	204118..205401	-	1284	WP_007246528.1	lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family protein	-
XJ28_RS01075	205556..206491	+	936	WP_007246529.1	LysR family transcriptional regulator	-
XJ28_RS01080	206740..207018	+	279	WP_007246530.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
XJ28_RS01085	207028..207333	+	306	WP_005739893.1	HigA family addiction module antidote protein	Antitoxin
XJ28_RS01090	207367..207648	-	282	WP_007246531.1	accessory factor UbiK family protein	-
XJ28_RS01100	208068..208406	+	339	WP_002555808.1	P-II family nitrogen regulator	-
XJ28_RS01105	208442..209779	+	1338	WP_005739890.1	ammonium transporter	-
XJ28_RS01115	210022..210447	+	426	WP_007246532.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
XJ28_RS01120	210546..210878	+	333	WP_007246533.1	hypothetical protein	-
XJ28_RS01125	210953..211645	-	693	WP_007246534.1	HAD family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10457.10 Da
• Isoelectric Point: 9.1490

>T73399 WP_007246530.1 NZ_CP019871:206740-207018 [Pseudomonas syringae pv. tomato]
MIVSFKCVHTRYLFLQGKTRLWPSIKSVAERKLAMLDAATSILDLRSPPGNRLEALDGSRSGQYSVRINAQFRICFVWSI
SGPEDVEIVDYH

Nucleotide

• Length: 279 bp

>T73399 NZ_CP019871:206740-207018 [Pseudomonas syringae pv. tomato]
ATGATTGTGAGTTTTAAGTGCGTGCACACGAGATATCTCTTTTTACAAGGCAAGACGCGGCTGTGGCCATCGATTAAATC
TGTTGCCGAGCGCAAGCTCGCAATGCTTGATGCTGCTACCTCGATTTTGGATCTGCGCTCTCCTCCCGGCAATCGACTAG
AGGCGCTTGATGGAAGTCGTTCTGGCCAATACAGCGTACGTATAAACGCTCAATTCAGGATTTGCTTCGTTTGGAGCATC
AGCGGTCCCGAGGACGTAGAAATTGTCGATTATCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11158.01 Da
• Isoelectric Point: 7.7509

>AT73399 WP_005739893.1 NZ_CP019871:207028-207333 [Pseudomonas syringae pv. tomato]
MNKNGMRPVHPGEVLKEEYLEPMGLTAAALARALKVSTPTVNDIVLQRRGVSADVALRLAVCLETSPEFWLNLQLAYDLR
KAETEKGAQIREQVKCLAHCA

Nucleotide

• Length: 306 bp

>AT73399 NZ_CP019871:207028-207333 [Pseudomonas syringae pv. tomato]
ATGAACAAAAACGGAATGCGCCCCGTCCATCCAGGCGAGGTTCTGAAAGAGGAATATCTTGAGCCGATGGGGCTGACCGC
CGCAGCGTTGGCCAGGGCCCTTAAGGTTTCTACGCCGACGGTGAACGACATCGTGCTCCAGCGCAGAGGTGTGAGCGCTG
ATGTAGCGCTGCGTCTGGCTGTATGCCTGGAGACGAGTCCTGAGTTCTGGCTTAACCTGCAGCTTGCCTACGATCTGCGC
AAGGCAGAGACAGAGAAGGGCGCGCAGATTCGTGAGCAGGTCAAATGTCTGGCCCACTGCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0K8LYY6