Detailed information of TA system TA073389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/VapI(antitoxin)
• Location: 90387..91815
• Replicon: chromosome
• Accession: NZ_CP019867
• Organism: Dehalococcoides mccartyi strain KBDCA1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: B1777_RS00510
• Protein ID: WP_058316891.1
• Coordinates: 90387..90725 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B1777_RS00515
• Protein ID: WP_058316890.1
• Coordinates: 90718..91815 (+)
• Length: 366 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1777_RS00470	85724..86896	-	1173	WP_077997816.1	hypothetical protein	-
B1777_RS00475	87165..87710	-	546	WP_077997817.1	AAA family ATPase	-
B1777_RS00480	87714..88040	-	327	WP_077997818.1	hypothetical protein	-
B1777_RS00485	88041..88562	-	522	WP_149867362.1	hypothetical protein	-
B1777_RS00490	88612..88842	-	231	WP_077997820.1	hypothetical protein	-
B1777_RS00495	88842..89138	-	297	WP_058316897.1	hypothetical protein	-
B1777_RS00500	89138..89797	-	660	WP_077997821.1	hypothetical protein	-
B1777_RS00505	89885..90265	+	381	WP_077997822.1	hypothetical protein	-
B1777_RS00510	90387..90725	+	339	WP_058316891.1	hypothetical protein	Toxin
B1777_RS00515	90718..91815	+	1098	WP_058316890.1	HigA family addiction module antidote protein	Antitoxin
B1777_RS00520	92378..92686	-	309	WP_011308733.1	rubrerythrin	-
B1777_RS00525	92875..94020	-	1146	WP_077997823.1	NTP transferase domain-containing protein	-
B1777_RS00530	94021..94710	-	690	WP_011308735.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	81075..92007	10932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (16-89)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12816.04 Da
• Isoelectric Point: 8.8488

>T73389 WP_058316891.1 NZ_CP019867:90387-90725 [Dehalococcoides mccartyi]
MDIIFCNRKLASIFNSEKNLKIAYGPENCKKIMNRMAVLKAAYCLDEIPVKPPERRHELKGDRKGQLAVDLKHPCRLIFR
PTSNPLPLKTDGGLDLRSVKGIEILEVEDYHE

Nucleotide

• Length: 339 bp

>T73389 NZ_CP019867:90387-90725 [Dehalococcoides mccartyi]
ATGGATATTATCTTTTGCAATAGGAAACTCGCAAGTATATTCAATAGTGAGAAAAATCTGAAGATAGCGTATGGCCCTGA
AAATTGCAAGAAGATTATGAATAGAATGGCTGTATTAAAAGCGGCCTACTGTCTTGATGAGATACCTGTAAAACCCCCTG
AGCGTAGACATGAGCTTAAAGGTGACAGGAAAGGACAATTAGCAGTTGACCTCAAACATCCCTGTCGGCTTATATTCCGA
CCCACTAGTAATCCTCTGCCCCTAAAGACTGATGGGGGCTTAGATCTTAGGAGTGTAAAAGGAATAGAAATTTTAGAAGT
TGAGGATTATCATGAGTAA

Antitoxin

Protein

• Length: 366 a.a.
• Molecular weight: 41295.39 Da
• Isoelectric Point: 6.3981

>AT73389 WP_058316890.1 NZ_CP019867:90718-91815 [Dehalococcoides mccartyi]
MSNTKLYRYEPDYVAVPGEILEENLEAQNIKKVDLASRCGLSTKTISQIINGTASISPETALGFQKVLGVSANIWLNLEA
DYRLFLAQQDLYKRLSKSGAWLNKFPIKELINKGFIEAKESIAEKTDQLLVFFGVSDIESWELSYNKLEVAFRHSPSFKS
LPEAIATWLRIGEICAAKVKCKPFDKSQFSEALNAIRILTPEEPKIFEPRLKEMCAEAGVALVFLEELPGMHLSGATRWI
TKDKALIMLSLRHKSDDHFWFSFFHEAGHILSGGKKSIFLDESNSWEDPEEDQANNFASDLLIPKQEYESFLKDGKFTSS
SVTCFAQNLGIAPGIVVGRLQHEQVIGWNSLYRLKRRFRLVEVCS

Nucleotide

• Length: 1098 bp

>AT73389 NZ_CP019867:90718-91815 [Dehalococcoides mccartyi]
ATGAGTAATACAAAATTATATAGATATGAACCTGATTATGTTGCAGTTCCGGGCGAGATATTAGAAGAAAACCTTGAGGC
TCAAAATATCAAAAAAGTTGATTTGGCTTCAAGATGTGGTTTAAGCACTAAAACTATTAGTCAAATCATAAATGGTACTG
CATCAATTTCTCCGGAAACAGCCTTGGGTTTTCAAAAAGTACTTGGTGTTTCCGCCAATATTTGGCTTAATCTTGAGGCA
GATTATAGATTATTCCTGGCGCAACAAGATCTGTATAAGAGACTATCTAAGTCTGGTGCATGGCTTAACAAGTTTCCTAT
TAAAGAACTCATTAATAAAGGGTTTATAGAAGCTAAAGAAAGTATTGCTGAAAAAACAGACCAATTGCTTGTATTTTTCG
GAGTAAGCGACATAGAATCTTGGGAATTAAGCTATAACAAGTTGGAAGTTGCATTTAGGCATTCTCCTTCATTTAAAAGT
TTGCCAGAAGCTATTGCTACATGGTTAAGAATTGGAGAAATCTGCGCTGCAAAAGTGAAATGTAAACCCTTTGATAAATC
TCAATTTTCAGAGGCACTCAACGCTATACGCATCCTTACTCCTGAAGAACCAAAGATTTTTGAGCCCCGCTTGAAAGAAA
TGTGTGCAGAAGCCGGTGTTGCCTTGGTATTTCTAGAGGAATTACCTGGTATGCATTTGAGTGGTGCAACGCGATGGATA
ACGAAAGATAAAGCATTAATAATGCTTAGTCTTAGACATAAAAGTGATGACCATTTCTGGTTTAGTTTTTTTCATGAAGC
TGGTCATATATTATCTGGTGGCAAGAAGAGTATATTCCTCGATGAATCAAATTCATGGGAAGATCCCGAAGAAGATCAAG
CAAACAATTTTGCTTCCGATCTATTGATACCCAAACAGGAATATGAGTCTTTTTTAAAAGACGGTAAGTTCACATCATCT
TCTGTCACATGCTTTGCACAAAATTTGGGAATTGCGCCCGGAATAGTTGTAGGACGTTTACAACACGAACAAGTTATTGG
ATGGAATTCTTTGTATCGCTTAAAAAGGAGATTTAGATTGGTTGAGGTTTGTAGTTAA