Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2381644..2382146 Replicon chromosome
Accession NZ_CP019778
Organism Escherichia coli strain NCTC86

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag B0915_RS12450 Protein ID WP_000767829.1
Coordinates 2381644..2381898 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag B0915_RS12455 Protein ID WP_001259255.1
Coordinates 2381895..2382146 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0915_RS12425 2376659..2376886 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
B0915_RS12430 2376900..2377958 - 1059 WP_078046021.1 transport protein YeeE -
B0915_RS12435 2378137..2379495 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
B0915_RS26585 2379485..2379547 - 63 WP_010723108.1 membrane protein YoeI -
B0915_RS12440 2379762..2380691 - 930 WP_000803360.1 LysR family transcriptional regulator -
B0915_RS12445 2380737..2381561 - 825 WP_000754762.1 SDR family oxidoreductase -
B0915_RS12450 2381644..2381898 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
B0915_RS12455 2381895..2382146 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
B0915_RS12460 2382429..2382479 + 51 WP_001364200.1 his operon leader peptide -
B0915_RS12470 2382625..2383524 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
B0915_RS12475 2383530..2384828 + 1299 WP_001743908.1 histidinol dehydrogenase -
B0915_RS12480 2384831..2385901 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
B0915_RS12485 2385901..2386968 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T73285 WP_000767829.1 NZ_CP019778:c2381898-2381644 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T73285 NZ_CP019778:c2381898-2381644 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT73285 WP_001259255.1 NZ_CP019778:c2382146-2381895 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT73285 NZ_CP019778:c2382146-2381895 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References