Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 286398..287092 Replicon chromosome
Accession NZ_CP019778
Organism Escherichia coli strain NCTC86

Toxin (Protein)


Gene name yafO Uniprot ID A0A3P5GWS5
Locus tag B0915_RS01335 Protein ID WP_001263488.1
Coordinates 286694..287092 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag B0915_RS01330 Protein ID WP_000554758.1
Coordinates 286398..286691 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0915_RS01310 282029..282527 + 499 Protein_245 REP-associated tyrosine transposase RayT -
B0915_RS01315 282751..284463 - 1713 Protein_246 flagellar biosynthesis protein FlhA -
B0915_RS01320 284435..285220 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
B0915_RS01325 285291..286346 + 1056 WP_001226164.1 DNA polymerase IV -
B0915_RS01330 286398..286691 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
B0915_RS01335 286694..287092 + 399 WP_001263488.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
B0915_RS01340 287102..287554 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
B0915_RS01345 287872..288078 + 207 Protein_252 RtcB family protein -
B0915_RS01350 288074..288595 + 522 Protein_253 peptide chain release factor H -
B0915_RS01355 288652..290109 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
B0915_RS01360 290370..290828 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 291424..291504 + 81 NuclAT_12 - -
- 291424..291504 + 81 NuclAT_12 - -
- 291424..291504 + 81 NuclAT_12 - -
- 291424..291504 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15559.90 Da        Isoelectric Point: 6.9798

>T73268 WP_001263488.1 NZ_CP019778:286694-287092 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQEAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T73268 NZ_CP019778:286694-287092 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGAGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT73268 WP_000554758.1 NZ_CP019778:286398-286691 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT73268 NZ_CP019778:286398-286691 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P5GWS5

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References