Detailed information of TA system TA073264

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4329781..4330383
• Replicon: chromosome
• Accession: NZ_CP019777
• Organism: Escherichia coli NU14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: B0908_RS22260
• Protein ID: WP_000897302.1
• Coordinates: 4329781..4330092 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: B0908_RS22265
• Protein ID: WP_000356397.1
• Coordinates: 4330093..4330383 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0908_RS22235	4325695..4326294	+	600	WP_001314324.1	glucose-1-phosphatase	-
B0908_RS22240	4326288..4327160	+	873	WP_000920762.1	virulence factor BrkB family protein	-
B0908_RS22245	4327157..4327594	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
B0908_RS22250	4327639..4328580	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
B0908_RS22255	4328644..4329552	-	909	WP_001331553.1	alpha/beta hydrolase	-
B0908_RS22260	4329781..4330092	+	312	WP_000897302.1	hypothetical protein	Toxin
B0908_RS22265	4330093..4330383	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
B0908_RS22275	4330742..4331020	+	279	WP_001296612.1	hypothetical protein	-
B0908_RS22285	4331416..4331634	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
B0908_RS22290	4331850..4332779	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
B0908_RS22295	4332776..4333411	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
B0908_RS22300	4333408..4334310	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T73264 WP_000897302.1 NZ_CP019777:4329781-4330092 [Escherichia coli NU14]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T73264 NZ_CP019777:4329781-4330092 [Escherichia coli NU14]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT73264 WP_000356397.1 NZ_CP019777:4330093-4330383 [Escherichia coli NU14]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT73264 NZ_CP019777:4330093-4330383 [Escherichia coli NU14]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416