Detailed information of TA system TA073222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 202480..203073
• Replicon: chromosome
• Accession: NZ_CP019730
• Organism: Pseudomonas syringae pv. actinidiae strain CRAFRU 12.29

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A2G7PD43
• Locus tag: B1R35_RS01020
• Protein ID: WP_003377943.1
• Coordinates: 202480..202758 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: Q88B19
• Locus tag: B1R35_RS01025
• Protein ID: WP_005739893.1
• Coordinates: 202768..203073 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B1R35_RS01000	197632..198465	-	834	WP_005739898.1	transporter substrate-binding domain-containing protein	-
B1R35_RS01005	198493..199365	-	873	WP_005613618.1	phytanoyl-CoA dioxygenase family protein	-
B1R35_RS01010	199390..200910	-	1521	WP_020313771.1	amino acid ABC transporter permease/ATP-binding protein	-
B1R35_RS01015	201395..202309	+	915	WP_019332092.1	LysR family transcriptional regulator	-
B1R35_RS01020	202480..202758	+	279	WP_003377943.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B1R35_RS01025	202768..203073	+	306	WP_005739893.1	HigA family addiction module antidote protein	Antitoxin
B1R35_RS01030	203107..203388	-	282	WP_017682769.1	accessory factor UbiK family protein	-
B1R35_RS01040	203808..204146	+	339	WP_002555808.1	P-II family nitrogen regulator	-
B1R35_RS01045	204181..205518	+	1338	WP_003377937.1	ammonium transporter	-
B1R35_RS01050	205506..205769	-	264	WP_017699472.1	hypothetical protein	-
B1R35_RS01055	205761..206186	+	426	WP_003377936.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
B1R35_RS01060	206285..206617	+	333	WP_003377934.1	hypothetical protein	-
B1R35_RS01065	206668..208005	-	1338	WP_032685517.1	IS4-like element ISPsy33 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	206668..208023	1355

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10512.18 Da
• Isoelectric Point: 9.1490

>T73222 WP_003377943.1 NZ_CP019730:202480-202758 [Pseudomonas syringae pv. actinidiae]
MIVSFKCVHTRYLFLQGKTRLWPSIKSVAERKLAMLDAATSILDLRSPPGNRLEVLDGSRSGQYSVRINAQFRICFVWSI
NGPEDVEIVDYH

Nucleotide

• Length: 279 bp

>T73222 NZ_CP019730:202480-202758 [Pseudomonas syringae pv. actinidiae]
ATGATTGTGAGTTTTAAGTGCGTGCACACGAGATATCTCTTTTTACAAGGCAAGACGCGGTTGTGGCCATCGATTAAATC
TGTTGCCGAGCGCAAGCTCGCAATGCTTGATGCTGCTACCTCGATTTTGGATCTGCGCTCTCCTCCCGGCAATCGACTAG
AGGTGCTTGATGGAAGTCGTTCTGGCCAATACAGCGTACGTATAAACGCTCAATTCAGGATTTGCTTCGTTTGGAGCATC
AACGGTCCCGAGGATGTAGAAATTGTCGATTATCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11158.01 Da
• Isoelectric Point: 7.7509

>AT73222 WP_005739893.1 NZ_CP019730:202768-203073 [Pseudomonas syringae pv. actinidiae]
MNKNGMRPVHPGEVLKEEYLEPMGLTAAALARALKVSTPTVNDIVLQRRGVSADVALRLAVCLETSPEFWLNLQLAYDLR
KAETEKGAQIREQVKCLAHCA

Nucleotide

• Length: 306 bp

>AT73222 NZ_CP019730:202768-203073 [Pseudomonas syringae pv. actinidiae]
ATGAACAAAAACGGAATGCGCCCCGTCCATCCAGGCGAGGTTCTGAAAGAGGAATATCTTGAGCCGATGGGGCTGACCGC
CGCAGCGTTGGCCAGAGCCCTTAAGGTTTCTACGCCGACGGTGAACGACATCGTGCTCCAGCGCAGAGGTGTGAGCGCTG
ATGTAGCGCTGCGTCTGGCTGTATGCCTGGAGACGAGTCCTGAGTTCTGGCTTAACCTGCAGCTTGCCTACGATCTGCGC
AAGGCAGAGACAGAGAAGGGCGCGCAGATTCGTGAGCAGGTCAAATGTCTGGCCCACTGCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A2G7PD43

Antitoxin

• AlphaFold DB: A0A0K8LYY6