Detailed information of TA system TA073220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2867788..2868335
• Replicon: chromosome
• Accession: NZ_CP019725
• Organism: Xanthomonas perforans 91-118

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: XPE_RS12895
• Protein ID: WP_074053292.1
• Coordinates: 2867788..2868027 (+)
• Length: 80 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: XPE_RS12900
• Protein ID: WP_008571772.1
• Coordinates: 2868045..2868335 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XPE_RS12890	2865315..2867690	+	2376	WP_046932680.1	AAA family ATPase	-
XPE_RS12895	2867788..2868027	+	240	WP_074053292.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
XPE_RS12900	2868045..2868335	+	291	WP_008571772.1	HigA family addiction module antidote protein	Antitoxin
XPE_RS12910	2868684..2870078	+	1395	WP_008571771.1	nucleoside hydrolase	-
XPE_RS22470	2870181..2870360	-	180	WP_003482327.1	hypothetical protein	-
XPE_RS12920	2870428..2872353	-	1926	WP_033478553.1	M61 family metallopeptidase	-
XPE_RS12930	2872689..2873021	-	333	WP_003482333.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-79)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-66)

Sequences

Toxin

Protein

• Length: 80 a.a.
• Molecular weight: 9270.65 Da
• Isoelectric Point: 9.9836

>T73220 WP_074053292.1 NZ_CP019725:2867788-2868027 [Xanthomonas perforans 91-118]
MGERSRRLPADIQLVARRTLRMLNAAAHLDNLRIPPANRLEASKGERPGQYSIRINDQWRICFRWMEGDVVQVEIVDYH

Nucleotide

• Length: 240 bp

>T73220 NZ_CP019725:2867788-2868027 [Xanthomonas perforans 91-118]
CTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCAATTGGTCGCGCGCCGCACGTTGCGCATGCTCAATGCCGCCGC
GCATCTCGACAATCTGCGGATTCCGCCCGCCAACCGGCTGGAAGCGTCGAAGGGCGAGCGGCCCGGCCAGTACAGCATCC
GGATCAACGATCAATGGCGCATCTGCTTCCGATGGATGGAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10337.97 Da
• Isoelectric Point: 6.4665

>AT73220 WP_008571772.1 NZ_CP019725:2868045-2868335 [Xanthomonas perforans 91-118]
MTVLPNIHPGEILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERVWLGLQADDELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT73220 NZ_CP019725:2868045-2868335 [Xanthomonas perforans 91-118]
ATGACGGTCCTACCCAATATCCACCCTGGCGAAATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGTCAGAACGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGAACCTCCGAGCGCGTCTGGCTTGGCCTGCAGGCCGACGACGAACTGGAGCAGGCG
CATCGCGCGCTGGGCGACTTGCCGGCGCGGATCAAGCGGCTGGCGGCATGA