Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2997789..2998330 Replicon chromosome
Accession NZ_CP019689
Organism Shigella sonnei strain 75/02

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag BZ172_RS16090 Protein ID WP_000615976.1
Coordinates 2997789..2998067 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag BZ172_RS16095 Protein ID WP_000729704.1
Coordinates 2998070..2998330 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BZ172_RS16075 (2995371) 2995371..2995949 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
BZ172_RS16080 (2996155) 2996155..2996922 + 768 WP_000333380.1 class II glutamine amidotransferase -
BZ172_RS16085 (2996893) 2996893..2997633 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
BZ172_RS16090 (2997789) 2997789..2998067 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
BZ172_RS16095 (2998070) 2998070..2998330 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
BZ172_RS16100 (2998516) 2998516..2999289 + 774 WP_127148796.1 C40 family peptidase -
BZ172_RS29260 (3000330) 3000330..3000458 + 129 Protein_2889 ISAs1 family transposase -
BZ172_RS16115 (3000529) 3000529..3001026 + 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
BZ172_RS16120 (3001149) 3001149..3002861 - 1713 Protein_2891 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 2987639..3001026 13387


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T73181 WP_000615976.1 NZ_CP019689:c2998067-2997789 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T73181 NZ_CP019689:c2998067-2997789 [Shigella sonnei]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT73181 WP_000729704.1 NZ_CP019689:c2998330-2998070 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT73181 NZ_CP019689:c2998330-2998070 [Shigella sonnei]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCTGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References