Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2089988..2090490 Replicon chromosome
Accession NZ_CP019629
Organism Escherichia coli strain MGY

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BWL12_RS11145 Protein ID WP_000767829.1
Coordinates 2089988..2090242 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BWL12_RS11150 Protein ID WP_001259255.1
Coordinates 2090239..2090490 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWL12_RS11120 2085003..2085230 - 228 WP_000234896.1 sulfurtransferase TusA -
BWL12_RS11125 2085244..2086302 - 1059 WP_000492339.1 transport protein YeeE -
BWL12_RS11130 2086481..2087839 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BWL12_RS24455 2087829..2087891 - 63 WP_010723108.1 membrane protein YoeI -
BWL12_RS11135 2088106..2089035 - 930 WP_000803351.1 LysR family transcriptional regulator -
BWL12_RS11140 2089081..2089905 - 825 WP_000754737.1 SDR family oxidoreductase -
BWL12_RS11145 2089988..2090242 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BWL12_RS11150 2090239..2090490 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BWL12_RS11155 2090773..2090823 + 51 WP_001364200.1 his operon leader peptide -
BWL12_RS11165 2090969..2091868 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
BWL12_RS11170 2091874..2093178 + 1305 WP_000009594.1 histidinol dehydrogenase -
BWL12_RS11175 2093175..2094245 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
BWL12_RS11180 2094245..2095312 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T73086 WP_000767829.1 NZ_CP019629:c2090242-2089988 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T73086 NZ_CP019629:c2090242-2089988 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT73086 WP_001259255.1 NZ_CP019629:c2090490-2090239 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT73086 NZ_CP019629:c2090490-2090239 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References