Detailed information of TA system TA073011

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1025943..1026613
• Replicon: chromosome
• Accession: NZ_CP019613
• Organism: Mycobacterium tuberculosis strain H112

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: B0W98_RS05225
• Protein ID: WP_003899954.1
• Coordinates: 1025943..1026287 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: B0W98_RS05230
• Protein ID: WP_003899955.1
• Coordinates: 1026284..1026613 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W98_RS05190	1021016..1021876	+	861	WP_003416628.1	alpha/beta hydrolase	-
B0W98_RS05195	1021971..1022366	+	396	WP_071854247.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
B0W98_RS05205	1022606..1022899	+	294	WP_003416635.1	hypothetical protein	-
B0W98_RS05210	1023187..1024476	+	1290	WP_003416640.1	ATP-binding protein	-
B0W98_RS05215	1024823..1025257	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
B0W98_RS05220	1025260..1025712	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
B0W98_RS05225	1025943..1026287	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B0W98_RS05230	1026284..1026613	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
B0W98_RS05235	1027151..1027498	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
B0W98_RS05240	1027495..1028115	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
B0W98_RS05245	1028275..1029540	-	1266	WP_003902423.1	hypothetical protein	-
B0W98_RS05250	1029708..1029917	+	210	WP_003416778.1	hypothetical protein	-
B0W98_RS05260	1030164..1031198	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T73011 WP_003899954.1 NZ_CP019613:1025943-1026287 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T73011 NZ_CP019613:1025943-1026287 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT73011 WP_003899955.1 NZ_CP019613:1026284-1026613 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT73011 NZ_CP019613:1026284-1026613 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCGGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6