73003

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 452642..453290 Replicon chromosome
Accession NZ_CP019613
Organism Mycobacterium tuberculosis strain H112

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag B0W98_RS02325 Protein ID WP_003899414.1
Coordinates 452642..452965 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag B0W98_RS02330 Protein ID WP_003899415.1
Coordinates 453045..453290 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W98_RS02295 447965..448963 + 999 WP_003900538.1 tyrosine-type recombinase/integrase -
B0W98_RS02300 448977..449441 + 465 WP_003900539.1 ParB N-terminal domain-containing protein -
B0W98_RS02305 449429..449680 + 252 WP_003908028.1 hypothetical protein -
B0W98_RS02310 449851..451290 - 1440 WP_003901443.1 phage major capsid protein -
B0W98_RS02315 451298..451831 - 534 WP_031666072.1 HK97 family phage prohead protease -
B0W98_RS02320 451984..452610 - 627 WP_003899413.1 phage terminase small subunit P27 family -
B0W98_RS02325 452642..452965 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
B0W98_RS02330 453045..453290 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
B0W98_RS02335 453287..454714 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
B0W98_RS02340 454716..455108 - 393 WP_003899417.1 DUF2742 domain-containing protein -
B0W98_RS02345 455105..455365 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
B0W98_RS02350 455382..455744 - 363 WP_003900543.1 hypothetical protein -
B0W98_RS02355 455747..456874 - 1128 WP_003899420.1 site-specific integrase -
B0W98_RS02360 457019..457246 - 228 WP_003899421.1 hypothetical protein -
B0W98_RS02365 457243..457632 - 390 WP_003899422.1 hypothetical protein -
B0W98_RS02370 457538..457810 + 273 WP_003900544.1 hypothetical protein -
B0W98_RS02375 457909..458142 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 447965..456874 8909


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-125)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-37)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T73003 WP_003899414.1 NZ_CP019613:c452965-452642 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T73003 NZ_CP019613:c452965-452642 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT73003 WP_003899415.1 NZ_CP019613:c453290-453045 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT73003 NZ_CP019613:c453290-453045 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References