Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4332958..4333628 Replicon chromosome
Accession NZ_CP019611
Organism Mycobacterium tuberculosis strain H83

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag B0W95_RS21070 Protein ID WP_003899954.1
Coordinates 4332958..4333302 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag B0W95_RS21075 Protein ID WP_003899955.1
Coordinates 4333299..4333628 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W95_RS21035 4328031..4328891 + 861 WP_003416628.1 alpha/beta hydrolase -
B0W95_RS21040 4328986..4329381 + 396 WP_071854247.1 nitroreductase family deazaflavin-dependent oxidoreductase -
B0W95_RS21050 4329621..4329914 + 294 WP_003416635.1 hypothetical protein -
B0W95_RS21055 4330202..4331491 + 1290 WP_003416640.1 ATP-binding protein -
B0W95_RS21060 4331838..4332272 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
B0W95_RS21065 4332275..4332727 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
B0W95_RS21070 4332958..4333302 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B0W95_RS21075 4333299..4333628 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
B0W95_RS21080 4334166..4334513 + 348 WP_003899956.1 DUF2384 domain-containing protein -
B0W95_RS21085 4334510..4335130 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
B0W95_RS21090 4335290..4336555 - 1266 WP_003899958.1 hypothetical protein -
B0W95_RS21095 4336723..4336932 + 210 WP_003416778.1 hypothetical protein -
B0W95_RS21105 4337179..4338213 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T72935 WP_003899954.1 NZ_CP019611:4332958-4333302 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T72935 NZ_CP019611:4332958-4333302 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT72935 WP_003899955.1 NZ_CP019611:4333299-4333628 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT72935 NZ_CP019611:4333299-4333628 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References