Detailed information of TA system TA072743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 1966841..1967264
• Replicon: chromosome
• Accession: NZ_CP019591
• Organism: Staphylococcus aureus strain 293G

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5S9I4Y6
• Locus tag: BXP64_RS09930
• Protein ID: WP_001065091.1
• Coordinates: 1967013..1967264 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0E0VQM8
• Locus tag: BXP64_RS09925
• Protein ID: WP_000028422.1
• Coordinates: 1966841..1967023 (-)
• Length: 61 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BXP64_RS09880	1962227..1963888	-	1662	WP_000625088.1	terminase large subunit	-
BXP64_RS09885	1963885..1964229	-	345	WP_000402904.1	hypothetical protein	-
BXP64_RS09890	1964359..1964658	-	300	WP_000988336.1	HNH endonuclease	-
BXP64_RS09895	1964890..1965306	-	417	WP_000590122.1	hypothetical protein	-
BXP64_RS09900	1965334..1965534	-	201	WP_000265043.1	DUF1514 family protein	-
BXP64_RS09905	1965534..1965683	-	150	WP_000595265.1	transcriptional activator RinB	-
BXP64_RS09910	1965680..1966066	-	387	WP_000592207.1	hypothetical protein	-
BXP64_RS09915	1966063..1966269	-	207	WP_000195803.1	DUF1381 domain-containing protein	-
BXP64_RS09920	1966306..1966848	-	543	WP_000185660.1	dUTP pyrophosphatase	-
BXP64_RS09925	1966841..1967023	-	183	WP_000028422.1	hypothetical protein	Antitoxin
BXP64_RS09930	1967013..1967264	-	252	WP_001065091.1	DUF1024 family protein	Toxin
BXP64_RS13910	1967257..1967430	-	174	WP_001793974.1	hypothetical protein	-
BXP64_RS09935	1967436..1967678	-	243	WP_000131366.1	hypothetical protein	-
BXP64_RS09940	1967682..1968050	-	369	WP_000101274.1	hypothetical protein	-
BXP64_RS09945	1968063..1968467	-	405	WP_000401960.1	RusA family crossover junction endodeoxyribonuclease	-
BXP64_RS09950	1968476..1968694	-	219	WP_000338530.1	hypothetical protein	-
BXP64_RS09955	1968701..1969585	-	885	WP_000148316.1	DnaD domain protein	-
BXP64_RS09960	1969615..1970085	-	471	WP_000934764.1	single-stranded DNA-binding protein	-
BXP64_RS09965	1970086..1970703	-	618	WP_071621397.1	MBL fold metallo-hydrolase	-
BXP64_RS09970	1970784..1971704	-	921	WP_000138472.1	recombinase RecT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	scn / chp / hlb / groEL	1941431..1995411	53980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9349.22 Da
• Isoelectric Point: 3.9473

>T72743 WP_001065091.1 NZ_CP019591:c1967264-1967013 [Staphylococcus aureus]
MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILEGMTNAIQHSVKEGVELDEAVGIMAGQVVYKYEEEQE
NEH

Nucleotide

• Length: 252 bp

>T72743 NZ_CP019591:c1967264-1967013 [Staphylococcus aureus]
ATGAATAATCGCGAACAAATTGAACAATCAGTTATAAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGATTGAGGACGTTTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGCATTAG

Antitoxin

Protein

• Length: 61 a.a.
• Molecular weight: 6741.63 Da
• Isoelectric Point: 4.2658

>AT72743 WP_000028422.1 NZ_CP019591:c1967023-1966841 [Staphylococcus aureus]
MSISVGDKVFNPETNSTLEIVQLVGDIRDTHYKLSDGSIISLIDFVVKPIHLIKEEQEND

Nucleotide

• Length: 183 bp

>AT72743 NZ_CP019591:c1967023-1966841 [Staphylococcus aureus]
ATGAGCATTAGTGTAGGAGACAAGGTTTTTAATCCAGAAACAAATTCAACTTTAGAAATTGTACAACTTGTTGGCGATAT
TAGAGACACGCATTACAAGTTATCTGACGGATCTATTATTAGTCTTATAGACTTTGTTGTTAAACCAATTCATTTAATCA
AGGAGGAGCAGGAAAATGACTAA

Structures

Toxin

• AlphaFold DB: A0A5S9I4Y6

Antitoxin

• AlphaFold DB: A0A0E0VQM8