Detailed information of TA system TA072740

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): holin-SprF3/-
• Location: 1944476..1944924
• Replicon: chromosome
• Accession: NZ_CP019591
• Organism: Staphylococcus aureus strain 293G

Toxin (Protein)

• Gene name: holin
• Uniprot ID: A0A853P973
• Locus tag: BXP64_RS09780
• Protein ID: WP_000387656.1
• Coordinates: 1944476..1944778 (-)
• Length: 101 a.a.

Antitoxin (RNA)

Gene name	SprF3
Locus tag	-
Coordinates	1944858..1944924 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BXP64_RS09745	1940059..1940306	+	248	Protein_1794	sphingomyelin phosphodiesterase	-
BXP64_RS09750	1940628..1940807	+	180	WP_000669789.1	hypothetical protein	-
BXP64_RS09760	1941118..1941378	+	261	WP_001791826.1	hypothetical protein	-
BXP64_RS09765	1941431..1941781	-	351	WP_000702263.1	complement inhibitor SCIN-A	-
BXP64_RS09770	1942466..1942915	+	450	WP_000727649.1	chemotaxis-inhibiting protein CHIPS	-
BXP64_RS09775	1943010..1944464	-	1455	WP_000930259.1	N-acetylmuramoyl-L-alanine amidase	-
BXP64_RS09780	1944476..1944778	-	303	WP_000387656.1	phage holin	Toxin
BXP64_RS09785	1944829..1944933	+	105	Protein_1801	hypothetical protein	-
-	1944858..1944924	+	67	NuclAT_0	-	Antitoxin
-	1944858..1944924	+	67	NuclAT_0	-	Antitoxin
-	1944858..1944924	+	67	NuclAT_0	-	Antitoxin
-	1944858..1944924	+	67	NuclAT_0	-	Antitoxin
BXP64_RS13945	1944977..1945124	-	148	Protein_1802	putative holin-like toxin	-
BXP64_RS09795	1945316..1945612	-	297	WP_000539688.1	DUF2951 domain-containing protein	-
BXP64_RS09800	1945670..1945957	-	288	WP_001040257.1	hypothetical protein	-
BXP64_RS09805	1946004..1946156	-	153	WP_001181555.1	hypothetical protein	-
BXP64_RS09810	1946143..1949823	-	3681	WP_000582140.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	scn / chp / hlb / groEL	1941431..1995411	53980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Holin (3-68)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11177.94 Da
• Isoelectric Point: 9.6587

>T72740 WP_000387656.1 NZ_CP019591:c1944778-1944476 [Staphylococcus aureus]
MEAKVMARYVVLILALVNQFLANKGISPIPVDEESISSIILTVIALYTTYKDNPTSQEGRWANQKLKKYKAESKYRKATG
QAPIKEVMTPTNMNDTNDLG

Nucleotide

• Length: 303 bp

>T72740 NZ_CP019591:c1944778-1944476 [Staphylococcus aureus]
ATGGAAGCAAAAGTAATGGCAAGATACGTTGTATTGATTTTAGCGTTAGTCAATCAGTTTTTAGCGAATAAAGGTATAAG
CCCGATACCAGTAGATGAAGAAAGTATTTCATCGATCATCTTAACAGTTATTGCTTTATACACTACGTATAAAGACAATC
CAACATCACAAGAAGGACGTTGGGCGAATCAAAAATTAAAGAAATATAAAGCAGAAAGTAAATATAGAAAAGCAACAGGA
CAAGCACCTATCAAAGAAGTGATGACACCTACAAATATGAACGACACAAATGATTTAGGGTAG

Antitoxin

Nucleotide

• Length: 67 bp

>AT72740 NZ_CP019591:1944858-1944924 [Staphylococcus aureus]
ATAAATAGAAAAAGGGCAACATGCGGAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCT

Structures

Toxin

• AlphaFold DB: A0A853P973

Antitoxin