Detailed information of TA system TA072705

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1115030..1115648
• Replicon: chromosome
• Accession: NZ_CP019584
• Organism: Sinorhizobium meliloti strain CCMM B554 (FSM-MA)

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: SMB554_RS05435
• Protein ID: WP_010969011.1
• Coordinates: 1115352..1115648 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SMB554_RS05430
• Protein ID: WP_010969010.1
• Coordinates: 1115030..1115338 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMB554_RS05385	1110109..1110429	-	321	WP_003527320.1	YnfA family protein	-
SMB554_RS05390	1110504..1110677	-	174	WP_010969006.1	hypothetical protein	-
SMB554_RS05395	1110691..1110864	-	174	WP_003527318.1	hypothetical protein	-
SMB554_RS05400	1111100..1111801	+	702	WP_010969007.1	glutathione S-transferase N-terminal domain-containing protein	-
SMB554_RS05405	1112025..1112366	-	342	WP_010969008.1	DUF2147 domain-containing protein	-
SMB554_RS05410	1112951..1113217	+	267	WP_003527312.1	sel1 repeat family protein	-
SMB554_RS05415	1113239..1114360	-	1122	WP_010969009.1	pyridoxal phosphate-dependent aminotransferase	-
SMB554_RS05425	1114789..1115019	-	231	WP_026029805.1	hypothetical protein	-
SMB554_RS05430	1115030..1115338	-	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
SMB554_RS05435	1115352..1115648	-	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SMB554_RS05440	1115696..1116001	-	306	WP_003527305.1	hypothetical protein	-
SMB554_RS05445	1116329..1117366	+	1038	WP_003527304.1	porin	-
SMB554_RS05450	1117476..1118543	-	1068	WP_014526650.1	site-specific integrase	-
SMB554_RS05455	1118584..1119633	-	1050	WP_010969013.1	porin	-
SMB554_RS05460	1119847..1120335	-	489	WP_013844230.1	BA14K family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T72705 WP_010969011.1 NZ_CP019584:c1115648-1115352 [Sinorhizobium meliloti]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T72705 NZ_CP019584:c1115648-1115352 [Sinorhizobium meliloti]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT72705 WP_010969010.1 NZ_CP019584:c1115338-1115030 [Sinorhizobium meliloti]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT72705 NZ_CP019584:c1115338-1115030 [Sinorhizobium meliloti]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61