Detailed information of TA system TA072657

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 75696..75965
• Replicon: plasmid pMRGN1031
• Accession: NZ_CP019561
• Organism: Escherichia coli strain KSC1031

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: BZY78_RS24595
• Protein ID: WP_001312861.1
• Coordinates: 75807..75965 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	75696..75761 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BZY78_RS25745	71231..71437	+	207	WP_000275853.1	hypothetical protein	-
BZY78_RS24570	71463..72002	+	540	WP_000290838.1	single-stranded DNA-binding protein	-
BZY78_RS24575	72065..72298	+	234	WP_000005990.1	DUF905 family protein	-
BZY78_RS24580	72364..74322	+	1959	WP_047402320.1	ParB/RepB/Spo0J family partition protein	-
BZY78_RS24585	74377..74811	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
BZY78_RS24590	74808..75527	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
BZY78_RS25605	75539..75727	-	189	WP_001299721.1	hypothetical protein	-
-	75539..75763	+	225	NuclAT_0	-	-
-	75539..75763	+	225	NuclAT_0	-	-
-	75539..75763	+	225	NuclAT_0	-	-
-	75539..75763	+	225	NuclAT_0	-	-
-	75696..75761	+	66	-	-	Antitoxin
BZY78_RS24595	75807..75965	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
BZY78_RS24615	77010..78014	-	1005	WP_024257664.1	IS110 family transposase	-
BZY78_RS24620	78265..78552	+	288	WP_000107548.1	hypothetical protein	-
BZY78_RS24625	78671..79492	+	822	WP_001234469.1	DUF945 domain-containing protein	-
BZY78_RS24630	79789..80391	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A)	vat	1..148529	148529

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T72657 WP_001312861.1 NZ_CP019561:75807-75965 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T72657 NZ_CP019561:75807-75965 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT72657 NZ_CP019561:75696-75761 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin