Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4233686..4234227 Replicon chromosome
Accession NZ_CP019560
Organism Escherichia coli strain KSC1031

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag BZY78_RS21620 Protein ID WP_000615976.1
Coordinates 4233686..4233964 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag BZY78_RS21625 Protein ID WP_000729704.1
Coordinates 4233967..4234227 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BZY78_RS21605 4231268..4231846 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
BZY78_RS21610 4232052..4232819 + 768 WP_000333380.1 class II glutamine amidotransferase -
BZY78_RS21615 4232790..4233530 - 741 WP_001225679.1 murein L,D-transpeptidase -
BZY78_RS21620 4233686..4233964 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
BZY78_RS21625 4233967..4234227 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
BZY78_RS21630 4234413..4235186 + 774 WP_000543899.1 C40 family peptidase -
BZY78_RS21635 4235243..4235599 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
BZY78_RS21640 4235592..4235870 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
BZY78_RS21645 4235975..4237687 - 1713 Protein_3962 flagellar biosynthesis protein FlhA -
BZY78_RS21650 4237659..4238444 + 786 WP_000207567.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4224558..4235870 11312


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T72652 WP_000615976.1 NZ_CP019560:c4233964-4233686 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T72652 NZ_CP019560:c4233964-4233686 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT72652 WP_000729704.1 NZ_CP019560:c4234227-4233967 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT72652 NZ_CP019560:c4234227-4233967 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References