Detailed information of TA system TA072649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3418609..3419211
• Replicon: chromosome
• Accession: NZ_CP019560
• Organism: Escherichia coli strain KSC1031

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: BZY78_RS17610
• Protein ID: WP_000897305.1
• Coordinates: 3418609..3418920 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BZY78_RS17615
• Protein ID: WP_000356397.1
• Coordinates: 3418921..3419211 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BZY78_RS17580	3413639..3414424	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
BZY78_RS17585	3414523..3415122	+	600	WP_001295269.1	glucose-1-phosphatase	-
BZY78_RS17590	3415116..3415988	+	873	WP_000920770.1	virulence factor BrkB family protein	-
BZY78_RS17595	3415985..3416422	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BZY78_RS17600	3416467..3417408	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
BZY78_RS17605	3417472..3418380	-	909	WP_001299484.1	alpha/beta hydrolase	-
BZY78_RS17610	3418609..3418920	+	312	WP_000897305.1	hypothetical protein	Toxin
BZY78_RS17615	3418921..3419211	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BZY78_RS17625	3419797..3420015	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
BZY78_RS17630	3420234..3420476	+	243	WP_001086388.1	hypothetical protein	-
BZY78_RS17635	3420705..3421685	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
BZY78_RS17645	3422084..3423013	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
BZY78_RS17650	3423010..3423645	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3420705..3421685	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T72649 WP_000897305.1 NZ_CP019560:3418609-3418920 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T72649 NZ_CP019560:3418609-3418920 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT72649 WP_000356397.1 NZ_CP019560:3418921-3419211 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT72649 NZ_CP019560:3418921-3419211 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7