Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1359254..1359756 Replicon chromosome
Accession NZ_CP019560
Organism Escherichia coli strain KSC1031

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BZY78_RS07135 Protein ID WP_000767829.1
Coordinates 1359254..1359508 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BZY78_RS07140 Protein ID WP_001259255.1
Coordinates 1359505..1359756 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BZY78_RS07110 1354269..1354496 - 228 WP_000985280.1 sulfurtransferase TusA family protein -
BZY78_RS07115 1354510..1355568 - 1059 WP_000492346.1 transport protein YeeE -
BZY78_RS07120 1355747..1357105 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BZY78_RS25115 1357095..1357157 - 63 WP_010723108.1 membrane protein YoeI -
BZY78_RS07125 1357372..1358301 - 930 WP_000803351.1 LysR family transcriptional regulator -
BZY78_RS07130 1358347..1359171 - 825 WP_000754752.1 SDR family oxidoreductase -
BZY78_RS07135 1359254..1359508 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BZY78_RS07140 1359505..1359756 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BZY78_RS07145 1360040..1360090 + 51 WP_001364200.1 his operon leader peptide -
BZY78_RS07155 1360236..1361135 + 900 WP_000566782.1 ATP phosphoribosyltransferase -
BZY78_RS07160 1361141..1362445 + 1305 WP_001342580.1 histidinol dehydrogenase -
BZY78_RS07165 1362442..1363512 + 1071 WP_000108992.1 histidinol-phosphate transaminase -
BZY78_RS07170 1363512..1364579 + 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T72643 WP_000767829.1 NZ_CP019560:c1359508-1359254 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T72643 NZ_CP019560:c1359508-1359254 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT72643 WP_001259255.1 NZ_CP019560:c1359756-1359505 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT72643 NZ_CP019560:c1359756-1359505 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References