Detailed information of TA system TA072625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4725147..4725736
• Replicon: chromosome
• Accession: NZ_CP019516
• Organism: Xanthomonas oryzae pv. oryzae strain CR1-5

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: BXU04_RS22125
• Protein ID: WP_012443754.1
• Coordinates: 4725455..4725736 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BXU04_RS22120
• Protein ID: WP_011260746.1
• Coordinates: 4725147..4725437 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BXU04_RS22090 (BXU04_23640)	4720291..4720908	-	618	WP_082325600.1	DUF4123 domain-containing protein	-
BXU04_RS22095 (BXU04_23645)	4720911..4721333	-	423	WP_042465811.1	PAAR domain-containing protein	-
BXU04_RS22100 (BXU04_23650)	4721461..4721787	-	327	WP_228329596.1	hypothetical protein	-
BXU04_RS22105 (BXU04_23660)	4722042..4722374	+	333	WP_011260743.1	hypothetical protein	-
BXU04_RS22110	4722534..4722721	+	188	Protein_4227	hypothetical protein	-
BXU04_RS22115 (BXU04_23665)	4722824..4724218	-	1395	WP_027703652.1	type III secretion system effector XopQ	-
BXU04_RS22120 (BXU04_23675)	4725147..4725437	-	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
BXU04_RS22125 (BXU04_23680)	4725455..4725736	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BXU04_RS22130 (BXU04_23685)	4725831..4728083	-	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T72625 WP_012443754.1 NZ_CP019516:c4725736-4725455 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T72625 NZ_CP019516:c4725736-4725455 [Xanthomonas oryzae pv. oryzae]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCACATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT72625 WP_011260746.1 NZ_CP019516:c4725437-4725147 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT72625 NZ_CP019516:c4725437-4725147 [Xanthomonas oryzae pv. oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAATGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5