Detailed information of TA system TA072620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4749583..4750172
• Replicon: chromosome
• Accession: NZ_CP019515
• Organism: Xanthomonas oryzae pv. oryzae strain SK2-3

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: BXU03_RS23580
• Protein ID: WP_012443754.1
• Coordinates: 4749891..4750172 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BXU03_RS23575
• Protein ID: WP_011260746.1
• Coordinates: 4749583..4749873 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BXU03_RS23535	4744868..4745344	-	477	WP_011260741.1	DUF4123 domain-containing protein	-
BXU03_RS23540	4745347..4745781	-	435	WP_011260742.1	PAAR domain-containing protein	-
BXU03_RS23545	4745897..4746142	-	246	WP_075240798.1	hypothetical protein	-
BXU03_RS23555	4746478..4746810	+	333	WP_011260743.1	hypothetical protein	-
BXU03_RS23565	4747260..4748654	-	1395	WP_075240796.1	nucleoside hydrolase	-
BXU03_RS23575	4749583..4749873	-	291	WP_011260746.1	HigA family addiction module antidote protein	Antitoxin
BXU03_RS23580	4749891..4750172	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BXU03_RS23585	4750267..4752525	-	2259	WP_075240794.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T72620 WP_012443754.1 NZ_CP019515:c4750172-4749891 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T72620 NZ_CP019515:c4750172-4749891 [Xanthomonas oryzae pv. oryzae]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCACATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT72620 WP_011260746.1 NZ_CP019515:c4749873-4749583 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT72620 NZ_CP019515:c4749873-4749583 [Xanthomonas oryzae pv. oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAATGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGGGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5