Detailed information of TA system TA072555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1144959..1145577
• Replicon: chromosome
• Accession: NZ_CP019488
• Organism: Sinorhizobium meliloti strain B399

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: BWO90_RS19490
• Protein ID: WP_010969011.1
• Coordinates: 1145281..1145577 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BWO90_RS19485
• Protein ID: WP_010969010.1
• Coordinates: 1144959..1145267 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWO90_RS19440	1140038..1140358	-	321	WP_003527320.1	YnfA family protein	-
BWO90_RS19445	1140433..1140606	-	174	WP_010969006.1	hypothetical protein	-
BWO90_RS19450	1140620..1140793	-	174	WP_003527318.1	hypothetical protein	-
BWO90_RS19455	1141029..1141730	+	702	WP_010969007.1	glutathione S-transferase N-terminal domain-containing protein	-
BWO90_RS19460	1141954..1142295	-	342	WP_010969008.1	DUF2147 domain-containing protein	-
BWO90_RS19465	1142880..1143146	+	267	WP_003527312.1	sel1 repeat family protein	-
BWO90_RS19470	1143168..1144289	-	1122	WP_010969009.1	pyridoxal phosphate-dependent aminotransferase	-
BWO90_RS19480	1144718..1144948	-	231	WP_026029805.1	hypothetical protein	-
BWO90_RS19485	1144959..1145267	-	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
BWO90_RS19490	1145281..1145577	-	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BWO90_RS19495	1145625..1145930	-	306	WP_095919950.1	hypothetical protein	-
BWO90_RS19500	1146258..1147295	+	1038	WP_095919952.1	porin	-
BWO90_RS19505	1147405..1148472	-	1068	WP_164829675.1	site-specific integrase	-
BWO90_RS19510	1148601..1149571	+	971	WP_095919954.1	IS630 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T72555 WP_010969011.1 NZ_CP019488:c1145577-1145281 [Sinorhizobium meliloti]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T72555 NZ_CP019488:c1145577-1145281 [Sinorhizobium meliloti]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT72555 WP_010969010.1 NZ_CP019488:c1145267-1144959 [Sinorhizobium meliloti]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT72555 NZ_CP019488:c1145267-1144959 [Sinorhizobium meliloti]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61