Detailed information of TA system TA072540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1106797..1107415
• Replicon: chromosome
• Accession: NZ_CP019485
• Organism: Sinorhizobium meliloti strain B401

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: BWO76_RS19135
• Protein ID: WP_010969011.1
• Coordinates: 1107119..1107415 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BWO76_RS19130
• Protein ID: WP_010969010.1
• Coordinates: 1106797..1107105 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWO76_RS19085	1101876..1102196	-	321	WP_003527320.1	YnfA family protein	-
BWO76_RS19090	1102271..1102444	-	174	WP_010969006.1	hypothetical protein	-
BWO76_RS19095	1102458..1102631	-	174	WP_003527318.1	hypothetical protein	-
BWO76_RS19100	1102867..1103568	+	702	WP_010969007.1	glutathione S-transferase N-terminal domain-containing protein	-
BWO76_RS19105	1103792..1104133	-	342	WP_010969008.1	DUF2147 domain-containing protein	-
BWO76_RS19110	1104718..1104984	+	267	WP_003527312.1	sel1 repeat family protein	-
BWO76_RS19115	1105006..1106127	-	1122	WP_010969009.1	pyridoxal phosphate-dependent aminotransferase	-
BWO76_RS19125	1106556..1106786	-	231	WP_026029805.1	hypothetical protein	-
BWO76_RS19130	1106797..1107105	-	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
BWO76_RS19135	1107119..1107415	-	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BWO76_RS19140	1107463..1107768	-	306	WP_095919950.1	hypothetical protein	-
BWO76_RS19145	1108096..1109133	+	1038	WP_095926588.1	porin	-
BWO76_RS19150	1109243..1110310	-	1068	WP_164829675.1	site-specific integrase	-
BWO76_RS19155	1110437..1111383	+	947	WP_011970797.1	IS630-like element ISRm2011-2 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T72540 WP_010969011.1 NZ_CP019485:c1107415-1107119 [Sinorhizobium meliloti]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T72540 NZ_CP019485:c1107415-1107119 [Sinorhizobium meliloti]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT72540 WP_010969010.1 NZ_CP019485:c1107105-1106797 [Sinorhizobium meliloti]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT72540 NZ_CP019485:c1107105-1106797 [Sinorhizobium meliloti]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61