Detailed information of TA system TA072507

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 154764..155185
• Replicon: plasmid pFHI_NMBU_03_1
• Accession: NZ_CP019456
• Organism: Escherichia coli strain FHI_NMBU_03

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: BXO92_RS24735
• Protein ID: WP_001302184.1
• Coordinates: 154764..154922 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	154987..155185 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BXO92_RS24700	150567..150950	-	384	WP_021572424.1	relaxosome protein TraM	-
BXO92_RS24705	151283..151885	+	603	WP_077695337.1	transglycosylase SLT domain-containing protein	-
BXO92_RS24710	152181..153002	-	822	WP_021572426.1	DUF945 domain-containing protein	-
BXO92_RS24715	153121..153408	-	288	WP_021572427.1	hypothetical protein	-
BXO92_RS24725	153564..153866	-	303	WP_001272235.1	hypothetical protein	-
BXO92_RS25165	153890..154096	-	207	WP_000547969.1	hypothetical protein	-
BXO92_RS25170	154209..154526	+	318	WP_000820281.1	hypothetical protein	-
BXO92_RS24735	154764..154922	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	154987..155185	-	199	NuclAT_0	-	Antitoxin
-	154987..155185	-	199	NuclAT_0	-	Antitoxin
-	154987..155185	-	199	NuclAT_0	-	Antitoxin
-	154987..155185	-	199	NuclAT_0	-	Antitoxin
BXO92_RS24740	155197..155916	-	720	WP_021572429.1	plasmid SOS inhibition protein A	-
BXO92_RS24745	155913..156347	-	435	WP_021572430.1	conjugation system SOS inhibitor PsiB	-
BXO92_RS24750	156402..158360	-	1959	WP_021572431.1	ParB/RepB/Spo0J family partition protein	-
BXO92_RS24755	158424..158657	-	234	WP_000005988.1	DUF905 family protein	-
BXO92_RS24760	158720..159259	-	540	WP_000290811.1	single-stranded DNA-binding protein	-
BXO92_RS25175	159285..159491	-	207	WP_000547969.1	hypothetical protein	-
BXO92_RS24770	159561..159819	+	259	Protein_154	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	papH / papC / papD / hlyB / iroB / iroC / iroD / iroE / iroN / faeJ / faeI / faeH / faeF / faeF / faeE / faeD / faeC	1..159821	159821

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T72507 WP_001302184.1 NZ_CP019456:c154922-154764 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T72507 NZ_CP019456:c154922-154764 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT72507 NZ_CP019456:c155185-154987 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin