Detailed information of TA system TA072504

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 117158..117412
• Replicon: plasmid pFHI_NMBU_03_1
• Accession: NZ_CP019456
• Organism: Escherichia coli strain FHI_NMBU_03

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: BXO92_RS24505
• Protein ID: WP_001351576.1
• Coordinates: 117158..117364 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	117351..117412 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BXO92_RS24475	112780..113316	-	537	WP_050576192.1	type 1 fimbrial protein	-
BXO92_RS25155	114600..114755	-	156	WP_032145292.1	hypothetical protein	-
BXO92_RS24485	115456..116313	-	858	WP_021572483.1	incFII family plasmid replication initiator RepA	-
BXO92_RS24490	116306..116380	-	75	WP_032144212.1	RepA leader peptide Tap	-
BXO92_RS24500	116626..116874	-	249	WP_000083837.1	replication regulatory protein RepA	-
BXO92_RS24505	117158..117364	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	117351..117412	+	62	NuclAT_1	-	Antitoxin
-	117351..117412	+	62	NuclAT_1	-	Antitoxin
-	117351..117412	+	62	NuclAT_1	-	Antitoxin
-	117351..117412	+	62	NuclAT_1	-	Antitoxin
BXO92_RS25160	117669..117743	-	75	Protein_102	endonuclease	-
BXO92_RS24515	117989..118201	-	213	WP_001299730.1	ANR family transcriptional regulator	-
BXO92_RS24520	118337..118897	-	561	WP_001587438.1	fertility inhibition protein FinO	-
BXO92_RS24525	118952..119698	-	747	WP_000205759.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	papH / papC / papD / hlyB / iroB / iroC / iroD / iroE / iroN / faeJ / faeI / faeH / faeF / faeF / faeE / faeD / faeC	1..159821	159821

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T72504 WP_001351576.1 NZ_CP019456:c117364-117158 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T72504 NZ_CP019456:c117364-117158 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GCTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT72504 NZ_CP019456:117351-117412 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin